Stimulated emission depletion microscopy with a single depletion laser using five fluorochromes and fluorescence lifetime phasor separation

Abstract Stimulated emission depletion (STED) microscopy achieves super-resolution by exciting a diffraction-limited volume and then suppressing fluorescence in its outer parts by depletion. Multiple depletion lasers may introduce misalignment and bleaching. Hence, a single depletion wavelength is p...

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Bibliographic Details
Main Authors: Mariano Gonzalez Pisfil, Iliya Nadelson, Brigitte Bergner, Sonja Rottmeier, Andreas W. Thomae, Steffen Dietzel
Format: Article
Language:English
Published: Nature Portfolio 2022-08-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-022-17825-5