High percentage of blood-based T-cell receptor gamma V9-JP recombinations associated with amyotrophic lateral sclerosis: extensive retention of the JP KKIK amino acid motif
Exome and RNAseq files prepared from blood samples can be mined for adaptive immune receptor recombinations and thus for the complementarity determining region-3 (CDR3) amino acid (AA) sequences, important for antigen binding. In this report, the T-cell receptor gamma (TRG) recombinations were mined...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Open Exploration Publishing Inc.
2023-12-01
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| Series: | Exploration of Immunology |
| Subjects: | |
| Online Access: | https://www.explorationpub.com/Journals/ei/Article/1003124 |
| _version_ | 1797376572294955008 |
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| author | George Blanck Taha I. Huda Konrad J. Cios George Angelakakis Joanna J. Song |
| author_facet | George Blanck Taha I. Huda Konrad J. Cios George Angelakakis Joanna J. Song |
| author_sort | George Blanck |
| collection | DOAJ |
| description | Exome and RNAseq files prepared from blood samples can be mined for adaptive immune receptor recombinations and thus for the complementarity determining region-3 (CDR3) amino acid (AA) sequences, important for antigen binding. In this report, the T-cell receptor gamma (TRG) recombinations were mined from amyotrophic lateral sclerosis (ALS) blood sample exome and RNAseq files, mainly inspired by: (i) a high level of gamma-delta T-cells in Parkinson’s disease and (ii) TRG CDR3 AA features associated with a higher Braak stage in Alzheimer’s disease. Results indicated a high percentage of V9-JP recombinations from ALS blood sample genomics files, in comparison to TRG recombinations obtained from a large number of blood and other tissue samples not representing ALS. This result is discussed in the context of potential phospholipid sponging by adaptive immune receptors and potential impacts on membrane rigidity and amyloid development. |
| first_indexed | 2024-03-08T19:40:28Z |
| format | Article |
| id | doaj.art-7bf74cc9add64d8088e2abe76fe4496c |
| institution | Directory Open Access Journal |
| issn | 2768-6655 |
| language | English |
| last_indexed | 2024-03-08T19:40:28Z |
| publishDate | 2023-12-01 |
| publisher | Open Exploration Publishing Inc. |
| record_format | Article |
| series | Exploration of Immunology |
| spelling | doaj.art-7bf74cc9add64d8088e2abe76fe4496c2023-12-25T07:14:04ZengOpen Exploration Publishing Inc.Exploration of Immunology2768-66552023-12-013659860310.37349/ei.2023.00124High percentage of blood-based T-cell receptor gamma V9-JP recombinations associated with amyotrophic lateral sclerosis: extensive retention of the JP KKIK amino acid motifGeorge Blanck0https://orcid.org/0000-0003-1664-0996Taha I. Huda1Konrad J. Cios2George Angelakakis3Joanna J. Song4Department of Molecular Medicine, Morsani College of Medicine, University of South Florida, Tampa, FL 33612, USA; Department of Immunology, H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL 33612, USADepartment of Molecular Medicine, Morsani College of Medicine, University of South Florida, Tampa, FL 33612, USADepartment of Molecular Medicine, Morsani College of Medicine, University of South Florida, Tampa, FL 33612, USADepartment of Molecular Medicine, Morsani College of Medicine, University of South Florida, Tampa, FL 33612, USADepartment of Molecular Medicine, Morsani College of Medicine, University of South Florida, Tampa, FL 33612, USAExome and RNAseq files prepared from blood samples can be mined for adaptive immune receptor recombinations and thus for the complementarity determining region-3 (CDR3) amino acid (AA) sequences, important for antigen binding. In this report, the T-cell receptor gamma (TRG) recombinations were mined from amyotrophic lateral sclerosis (ALS) blood sample exome and RNAseq files, mainly inspired by: (i) a high level of gamma-delta T-cells in Parkinson’s disease and (ii) TRG CDR3 AA features associated with a higher Braak stage in Alzheimer’s disease. Results indicated a high percentage of V9-JP recombinations from ALS blood sample genomics files, in comparison to TRG recombinations obtained from a large number of blood and other tissue samples not representing ALS. This result is discussed in the context of potential phospholipid sponging by adaptive immune receptors and potential impacts on membrane rigidity and amyloid development.https://www.explorationpub.com/Journals/ei/Article/1003124amyotrophic lateral sclerosist-cell receptor gammaphospholipidsamyloid development |
| spellingShingle | George Blanck Taha I. Huda Konrad J. Cios George Angelakakis Joanna J. Song High percentage of blood-based T-cell receptor gamma V9-JP recombinations associated with amyotrophic lateral sclerosis: extensive retention of the JP KKIK amino acid motif Exploration of Immunology amyotrophic lateral sclerosis t-cell receptor gamma phospholipids amyloid development |
| title | High percentage of blood-based T-cell receptor gamma V9-JP recombinations associated with amyotrophic lateral sclerosis: extensive retention of the JP KKIK amino acid motif |
| title_full | High percentage of blood-based T-cell receptor gamma V9-JP recombinations associated with amyotrophic lateral sclerosis: extensive retention of the JP KKIK amino acid motif |
| title_fullStr | High percentage of blood-based T-cell receptor gamma V9-JP recombinations associated with amyotrophic lateral sclerosis: extensive retention of the JP KKIK amino acid motif |
| title_full_unstemmed | High percentage of blood-based T-cell receptor gamma V9-JP recombinations associated with amyotrophic lateral sclerosis: extensive retention of the JP KKIK amino acid motif |
| title_short | High percentage of blood-based T-cell receptor gamma V9-JP recombinations associated with amyotrophic lateral sclerosis: extensive retention of the JP KKIK amino acid motif |
| title_sort | high percentage of blood based t cell receptor gamma v9 jp recombinations associated with amyotrophic lateral sclerosis extensive retention of the jp kkik amino acid motif |
| topic | amyotrophic lateral sclerosis t-cell receptor gamma phospholipids amyloid development |
| url | https://www.explorationpub.com/Journals/ei/Article/1003124 |
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