An optimization of four SARS-CoV-2 qRT-PCR assays in a Kenyan laboratory to support the national COVID-19 rapid response teams [version 2; peer review: 2 approved]

An optimization of four SARS-CoV-2 qRT-PCR assays in a Kenyan laboratory to support the national COVID-19 rapid response teams [version 2; peer review: 2 approved]

Background: The COVID-19 pandemic relies on real-time polymerase chain reaction (qRT-PCR) for the detection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), to facilitate roll-out of patient care and infection control measures. There are several qRT-PCR assays with little evidence on...

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Bibliographic Details
Main Authors: Shadrack Mutua, Brian Bartilol, Shaban J. Mwangi, Debra Riako, Lydia Nyamako, Bonface M. Gichuki, Henry Karanja, Angela Karani, John N. Gitonga, Daisy Mugo, Brian Tawa, Wilson Gumbi, Wesley Cheruiyot, Metrine Tendwa, John K. Nyambu, Yiakon Sein, Thani Suleiman Thani, Shem O. Patta, Benson Kitole, Eric K. Maitha, Barke S. Muslih, Mohamed S. Mwakinangu, Philip Bejon, Benjamin Tsofa, Joyce U. Nyiro, John Ochieng Otieno, Leonard Ndwiga, Patience Kiyuka, Johnstone Makale, Kevin Wamae, Victor Osoti, John Mwita Morobe, Calleb Odundo, Arnold W. Lambisia, Martin Mutunga, Salim Mwarumba, Lynette Isabella Ochola-Oyier, Charles N. Agoti, Clement Lewa, Elijah Gicheru, Jennifer Musyoki, Susan Njuguna, Horace Gumba, Domtila Kimani, Jedidah Mwacharo, Zaydah R. de Laurent, Khadija Said Mohammed, Robinson Cheruiyot, Donwilliams O. Omuoyo
Format: Article
Language:English
Published: Wellcome 2022-03-01
Series:Wellcome Open Research
Subjects:
COVID-19
SARS-CoV-2
coronavirus
qRT-PCR
diagnosis
optimization
eng
Online Access:https://wellcomeopenresearch.org/articles/5-162/v2

Internet

https://wellcomeopenresearch.org/articles/5-162/v2

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