Protocol for imbibed seed piercing for Agrobacterium-mediated transformation of jute

Protocol for imbibed seed piercing for Agrobacterium-mediated transformation of jute

Summary: Here, we present a streamlined Agrobacterium-mediated transformation protocol for jute (Corchorus sp.). We describe steps to pierce and vacuum infiltrate imbibed jute seeds with Agrobacterium suspension. We then detail procedures for selecting transformed seeds by using a hygromycin-B-suppl...

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Bibliographic Details
Main Authors: Shuvobrata Majumder, Subhadarshini Parida, Nrisingha Dey
Format: Article
Language:English
Published: Elsevier 2024-03-01
Series:STAR Protocols
Subjects:
Genetics
Model Organisms
Plant sciences
Molecular Biology
Gene Expression
Biotechnology and bioengineering
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723007347

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http://www.sciencedirect.com/science/article/pii/S2666166723007347

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