Establishment and Optimization of Molecular Cytogenetic Techniques (45S rDNA-FISH, GISH, and Fiber-FISH) in Kiwifruit (Actinidia Lindl.)

Establishment and Optimization of Molecular Cytogenetic Techniques (45S rDNA-FISH, GISH, and Fiber-FISH) in Kiwifruit (Actinidia Lindl.)

The kiwifruit (Actinidia chinensis) has long been regarded as “the king of fruits” for its nutritional importance. However, the molecular cytogenetics of kiwifruit has long been hampered because of the large number of basic chromosome (x = 29), the inherent small size and highly similar morphology o...

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Bibliographic Details
Main Authors: Yang Zhao, Honghong Deng, Yao Chen, Jihan Li, Silei Chen, Chunyan Li, Xue Mu, Zhongrong Hu, Kunming Li, Weixing Wang
Format: Article
Language:English
Published: Frontiers Media S.A. 2022-06-01
Series:Frontiers in Plant Science
Subjects:
45S rDNA-FISH
blocking DNA
fiber-FISH
GISH
nuclei isolation
post-hybridization washing temperature
Online Access:https://www.frontiersin.org/articles/10.3389/fpls.2022.906168/full

Internet

https://www.frontiersin.org/articles/10.3389/fpls.2022.906168/full

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