Parameter optimization for stable clustering using FlowSOM: a case study from CyTOF

High-dimensional cell phenotyping is a powerful tool to study molecular and cellular changes in health and diseases. CyTOF enables high-dimensional cell phenotyping using tens of surface and intra-cellular markers. To utilize the full potential of CyTOF, we need advanced clustering and machine learn...

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Bibliographic Details
Main Authors: Weiyang Tao, Anirban Sinha, Khadir Raddassi, Aridaman Pandit
Format: Article
Language:English
Published: Frontiers Media S.A. 2024-10-01
Series:Frontiers in Immunology
Subjects:
Online Access:https://www.frontiersin.org/articles/10.3389/fimmu.2024.1414400/full