Dissociation of neonatal and adult mice brain for simultaneous analysis of microglia, astrocytes and infiltrating lymphocytes by flow cytometry

Dissociation of neonatal and adult mice brain for simultaneous analysis of microglia, astrocytes and infiltrating lymphocytes by flow cytometry

The technical difficulty to isolate microglia, astrocytes and infiltrating immune cells from mouse brain is nowadays a limiting factor in the study of neuroinflammation. Brain isolation requirements are cell-type and animal-age dependent, but current brain dissociation procedures are poorly standard...

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Bibliographic Details
Main Authors: Belén Calvo, Felipe Rubio, Miriam Fernández, Pedro Tranque
Format: Article
Language:English
Published: Elsevier 2020-06-01
Series:IBRO Reports
Subjects:
Microglia
Astrocytes
Glia reactivity
Lymphocytes
Neuroimmunity
Flow cytometry
Online Access:http://www.sciencedirect.com/science/article/pii/S2451830120300017

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http://www.sciencedirect.com/science/article/pii/S2451830120300017

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