Optimized single-cell RNA sequencing protocol to study early genome activation in mammalian preimplantation development

Optimized single-cell RNA sequencing protocol to study early genome activation in mammalian preimplantation development

Summary: Here, we present a modification of single-cell tagged reverse transcription protocol to study gene expression on a single-cell level or with limited RNA input. We describe different enzymes for reverse transcription and cDNA amplification, modified lysis buffer, and additional clean-up step...

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Bibliographic Details
Main Authors: Nina Boskovic, Gamze Yazgeldi, Sini Ezer, Mari H. Tervaniemi, Jose Inzunza, Spyridon Panagiotis Deligiannis, Barış Yaşar, Tiina Skoog, Kaarel Krjutškov, Shintaro Katayama, Juha Kere
Format: Article
Language:English
Published: Elsevier 2023-09-01
Series:STAR Protocols
Subjects:
Bioinformatics
Sequence analysis
Single Cell
Developmental biology
Genomics
Sequencing
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723003246

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http://www.sciencedirect.com/science/article/pii/S2666166723003246

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