A versatile genomic transgenesis platform with enhanced λ integrase for human Expi293F cells

A versatile genomic transgenesis platform with enhanced λ integrase for human Expi293F cells

Reliable cell-based platforms to test and/or produce biologics in a sustainable manner are important for the biotech industry. Utilizing enhanced λ integrase, a sequence-specific DNA recombinase, we developed a novel transgenesis platform involving a fully characterized single genomic locus as an ar...

Full description

Bibliographic Details
Main Authors: Asim Azhar Siddiqui, Sabrina Peter, Eve Zi Xian Ngoh, Cheng-I. Wang, Shirelle Ng, John A. Dangerfield, Walter H. Gunzburg, Peter Dröge, Harshyaa Makhija
Format: Article
Language:English
Published: Frontiers Media S.A. 2023-06-01
Series:Frontiers in Bioengineering and Biotechnology
Subjects:
human cell line engineering
λ integrase
Expi293F cells
biotherapeutics
site-specific transgenesis
microencapsulation
Online Access:https://www.frontiersin.org/articles/10.3389/fbioe.2023.1198465/full

Internet

https://www.frontiersin.org/articles/10.3389/fbioe.2023.1198465/full

Similar Items