Effect of Lyoprotective Agents on the Preservation of Survival of a <i>Bacillus cereus</i> Strain PBG in the Freeze-Drying Process
Lyophilization is a widely employed long-term preservation method in which the bacterial survival rate largely depends on the cryoprotectant used. <i>Bacillus cereus</i> strain PBC was selected for its ability to thrive in environments contaminated with arsenic, lead, and cadmium, tolera...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-11-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/11/2705 |
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| author | Diana Galeska Farfan Pajuelo Milena Carpio Mamani Gisela July Maraza Choque Dina Mayumi Chachaque Callo César Julio Cáceda Quiroz |
| author_facet | Diana Galeska Farfan Pajuelo Milena Carpio Mamani Gisela July Maraza Choque Dina Mayumi Chachaque Callo César Julio Cáceda Quiroz |
| author_sort | Diana Galeska Farfan Pajuelo |
| collection | DOAJ |
| description | Lyophilization is a widely employed long-term preservation method in which the bacterial survival rate largely depends on the cryoprotectant used. <i>Bacillus cereus</i> strain PBC was selected for its ability to thrive in environments contaminated with arsenic, lead, and cadmium, tolerate 500 ppm of free cyanide, and the presence of genes such as <i>ars</i>, <i>cad</i>, <i>ppa</i>, <i>dap</i>, among others, associated with the bioremediation of toxic compounds and enterotoxins (<i>nheA</i>, <i>nheB</i>, <i>nheC</i>). Following lyophilization, the survival rates for Mannitol 2.5%, Mannitol 10%, and Glucose 1% were 98.02%, 97.12%, and 96.30%, respectively, with the rates being lower than 95% for other sugars. However, during storage, for the same sugars, the survival rates were 78.71%, 97.12%, and 99.97%, respectively. In the cake morphology, it was found that the lyophilized morphology showed no relationship with bacterial survival rate. The best cryoprotectant for the PBC strain was 1% glucose since it maintained constant and elevated bacterial growth rates during storage, ensuring that the unique characteristics of the bacterium were preserved over time. These findings hold significant implications for research as they report a new <i>Bacillus cereus</i> strain with the potential to be utilized in bioremediation processes. |
| first_indexed | 2024-03-09T16:35:17Z |
| format | Article |
| id | doaj.art-7d26baa6752044ee8bd729eb13850494 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T16:35:17Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-7d26baa6752044ee8bd729eb138504942023-11-24T14:56:58ZengMDPI AGMicroorganisms2076-26072023-11-011111270510.3390/microorganisms11112705Effect of Lyoprotective Agents on the Preservation of Survival of a <i>Bacillus cereus</i> Strain PBG in the Freeze-Drying ProcessDiana Galeska Farfan Pajuelo0Milena Carpio Mamani1Gisela July Maraza Choque2Dina Mayumi Chachaque Callo3César Julio Cáceda Quiroz4Bioremediation Laboratory, Jorge Basadre Grohmann National University, Tacna 230001, PeruBioremediation Laboratory, Jorge Basadre Grohmann National University, Tacna 230001, PeruBioremediation Laboratory, Jorge Basadre Grohmann National University, Tacna 230001, PeruBioremediation Laboratory, Jorge Basadre Grohmann National University, Tacna 230001, PeruBioremediation Laboratory, Jorge Basadre Grohmann National University, Tacna 230001, PeruLyophilization is a widely employed long-term preservation method in which the bacterial survival rate largely depends on the cryoprotectant used. <i>Bacillus cereus</i> strain PBC was selected for its ability to thrive in environments contaminated with arsenic, lead, and cadmium, tolerate 500 ppm of free cyanide, and the presence of genes such as <i>ars</i>, <i>cad</i>, <i>ppa</i>, <i>dap</i>, among others, associated with the bioremediation of toxic compounds and enterotoxins (<i>nheA</i>, <i>nheB</i>, <i>nheC</i>). Following lyophilization, the survival rates for Mannitol 2.5%, Mannitol 10%, and Glucose 1% were 98.02%, 97.12%, and 96.30%, respectively, with the rates being lower than 95% for other sugars. However, during storage, for the same sugars, the survival rates were 78.71%, 97.12%, and 99.97%, respectively. In the cake morphology, it was found that the lyophilized morphology showed no relationship with bacterial survival rate. The best cryoprotectant for the PBC strain was 1% glucose since it maintained constant and elevated bacterial growth rates during storage, ensuring that the unique characteristics of the bacterium were preserved over time. These findings hold significant implications for research as they report a new <i>Bacillus cereus</i> strain with the potential to be utilized in bioremediation processes.https://www.mdpi.com/2076-2607/11/11/2705lyophilizationbacterial survivalmannitolglucoselactose and <i>Bacillus</i> |
| spellingShingle | Diana Galeska Farfan Pajuelo Milena Carpio Mamani Gisela July Maraza Choque Dina Mayumi Chachaque Callo César Julio Cáceda Quiroz Effect of Lyoprotective Agents on the Preservation of Survival of a <i>Bacillus cereus</i> Strain PBG in the Freeze-Drying Process Microorganisms lyophilization bacterial survival mannitol glucose lactose and <i>Bacillus</i> |
| title | Effect of Lyoprotective Agents on the Preservation of Survival of a <i>Bacillus cereus</i> Strain PBG in the Freeze-Drying Process |
| title_full | Effect of Lyoprotective Agents on the Preservation of Survival of a <i>Bacillus cereus</i> Strain PBG in the Freeze-Drying Process |
| title_fullStr | Effect of Lyoprotective Agents on the Preservation of Survival of a <i>Bacillus cereus</i> Strain PBG in the Freeze-Drying Process |
| title_full_unstemmed | Effect of Lyoprotective Agents on the Preservation of Survival of a <i>Bacillus cereus</i> Strain PBG in the Freeze-Drying Process |
| title_short | Effect of Lyoprotective Agents on the Preservation of Survival of a <i>Bacillus cereus</i> Strain PBG in the Freeze-Drying Process |
| title_sort | effect of lyoprotective agents on the preservation of survival of a i bacillus cereus i strain pbg in the freeze drying process |
| topic | lyophilization bacterial survival mannitol glucose lactose and <i>Bacillus</i> |
| url | https://www.mdpi.com/2076-2607/11/11/2705 |
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