A Method for Rapid Polyethyleneimine-Based Purification of Bacteriophage-Expressed Proteins from Diluted Crude Lysates, Exemplified by Thermostable TP-84 Depolymerase

A Method for Rapid Polyethyleneimine-Based Purification of Bacteriophage-Expressed Proteins from Diluted Crude Lysates, Exemplified by Thermostable TP-84 Depolymerase

Purification of bacteriophage-expressed proteins poses methodological difficulties associated with the need to process entire culture medium volume upon bacteriophage-induced bacterial cell lysis. We have used novel capsule glycosylase-depolymerase (TP84_26 GD) from bacteriophage TP-84, infecting th...

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Bibliographic Details
Main Authors: Beata Łubkowska, Edyta Czajkowska, Ireneusz Sobolewski, Natalia Krawczun, Agnieszka Żylicz-Stachula, Piotr M. Skowron
Format: Article
Language:English
Published: MDPI AG 2023-09-01
Series:Microorganisms
Subjects:
biotechnology
depolymerase
capsule
envelope
biofilm
biofilm removal
Online Access:https://www.mdpi.com/2076-2607/11/9/2340

Internet

https://www.mdpi.com/2076-2607/11/9/2340

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