Polyphenolic Extracts from Spent Coffee Grounds Prevent H<sub>2</sub>O<sub>2</sub>-Induced Oxidative Stress in <i>Centropomus viridis</i> Brain Cells
Oxidative stress in aquatic organisms might suppress the immune system and propagate infectious diseases. This study aimed to investigate the protective effect of polyphenolic extracts from spent coffee grounds (SCG) against oxidative stress, induced by H<sub>2</sub>O<sub>2</sub...
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| Format: | Article |
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MDPI AG
2021-10-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/20/6195 |
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| author | Nayely Leyva-López Melissa Peraza-Arias Anaguiven Avalos-Soriano Crisantema Hernández Cynthia E. Lizárraga-Velázquez J. Basilio Heredia |
| author_facet | Nayely Leyva-López Melissa Peraza-Arias Anaguiven Avalos-Soriano Crisantema Hernández Cynthia E. Lizárraga-Velázquez J. Basilio Heredia |
| author_sort | Nayely Leyva-López |
| collection | DOAJ |
| description | Oxidative stress in aquatic organisms might suppress the immune system and propagate infectious diseases. This study aimed to investigate the protective effect of polyphenolic extracts from spent coffee grounds (SCG) against oxidative stress, induced by H<sub>2</sub>O<sub>2</sub>, in <i>C. viridis</i> brain cells, through an in vitro model. Hydrophilic extracts from SCG are rich in quinic, ferulic and caffeic acids and showed antioxidant capacity in DPPH, ORAC and FRAP assays. Furthermore, pretreatment of <i>C. viridis</i> brain cells with the polyphenolic extracts from SCG (230 and 460 µg/mL) for 24 h prior to 100 µM H<sub>2</sub>O<sub>2</sub> exposure (1 h) significantly increased antioxidant enzymes activity (superoxide dismutase and catalase) and reduced lipid peroxidation (measured by MDA levels). These results suggest that polyphenols found in SCG extracts exert an antioxidative protective effect against oxidative stress in <i>C. viridis</i> brain cells by stimulating the activity of SOD and CAT. |
| first_indexed | 2024-03-10T06:21:09Z |
| format | Article |
| id | doaj.art-7d4558c542ba489fb96e02d4612ecb88 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T06:21:09Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-7d4558c542ba489fb96e02d4612ecb882023-11-22T19:19:35ZengMDPI AGMolecules1420-30492021-10-012620619510.3390/molecules26206195Polyphenolic Extracts from Spent Coffee Grounds Prevent H<sub>2</sub>O<sub>2</sub>-Induced Oxidative Stress in <i>Centropomus viridis</i> Brain CellsNayely Leyva-López0Melissa Peraza-Arias1Anaguiven Avalos-Soriano2Crisantema Hernández3Cynthia E. Lizárraga-Velázquez4J. Basilio Heredia5Centro de Investigación en Alimentación y Desarrollo, A.C., Av. Sábalo Cerritos S/N, Mazatlán 82112, Sinaloa, MexicoCentro de Investigación en Alimentación y Desarrollo, A.C., Av. Sábalo Cerritos S/N, Mazatlán 82112, Sinaloa, MexicoCentro de Investigación en Alimentación y Desarrollo, A.C., Av. Sábalo Cerritos S/N, Mazatlán 82112, Sinaloa, MexicoCentro de Investigación en Alimentación y Desarrollo, A.C., Av. Sábalo Cerritos S/N, Mazatlán 82112, Sinaloa, MexicoCentro de Investigación en Alimentación y Desarrollo, A.C., Av. Sábalo Cerritos S/N, Mazatlán 82112, Sinaloa, MexicoCentro de Investigación en Alimentación y Desarrollo, A.C., Carretera a Eldorado Km. 5.5, Col. Campo El Diez, Culiacán 80110, Sinaloa, MexicoOxidative stress in aquatic organisms might suppress the immune system and propagate infectious diseases. This study aimed to investigate the protective effect of polyphenolic extracts from spent coffee grounds (SCG) against oxidative stress, induced by H<sub>2</sub>O<sub>2</sub>, in <i>C. viridis</i> brain cells, through an in vitro model. Hydrophilic extracts from SCG are rich in quinic, ferulic and caffeic acids and showed antioxidant capacity in DPPH, ORAC and FRAP assays. Furthermore, pretreatment of <i>C. viridis</i> brain cells with the polyphenolic extracts from SCG (230 and 460 µg/mL) for 24 h prior to 100 µM H<sub>2</sub>O<sub>2</sub> exposure (1 h) significantly increased antioxidant enzymes activity (superoxide dismutase and catalase) and reduced lipid peroxidation (measured by MDA levels). These results suggest that polyphenols found in SCG extracts exert an antioxidative protective effect against oxidative stress in <i>C. viridis</i> brain cells by stimulating the activity of SOD and CAT.https://www.mdpi.com/1420-3049/26/20/6195antioxidant capacitycatalasesuperoxide dismutaselipid peroxidationwhite snook |
| spellingShingle | Nayely Leyva-López Melissa Peraza-Arias Anaguiven Avalos-Soriano Crisantema Hernández Cynthia E. Lizárraga-Velázquez J. Basilio Heredia Polyphenolic Extracts from Spent Coffee Grounds Prevent H<sub>2</sub>O<sub>2</sub>-Induced Oxidative Stress in <i>Centropomus viridis</i> Brain Cells Molecules antioxidant capacity catalase superoxide dismutase lipid peroxidation white snook |
| title | Polyphenolic Extracts from Spent Coffee Grounds Prevent H<sub>2</sub>O<sub>2</sub>-Induced Oxidative Stress in <i>Centropomus viridis</i> Brain Cells |
| title_full | Polyphenolic Extracts from Spent Coffee Grounds Prevent H<sub>2</sub>O<sub>2</sub>-Induced Oxidative Stress in <i>Centropomus viridis</i> Brain Cells |
| title_fullStr | Polyphenolic Extracts from Spent Coffee Grounds Prevent H<sub>2</sub>O<sub>2</sub>-Induced Oxidative Stress in <i>Centropomus viridis</i> Brain Cells |
| title_full_unstemmed | Polyphenolic Extracts from Spent Coffee Grounds Prevent H<sub>2</sub>O<sub>2</sub>-Induced Oxidative Stress in <i>Centropomus viridis</i> Brain Cells |
| title_short | Polyphenolic Extracts from Spent Coffee Grounds Prevent H<sub>2</sub>O<sub>2</sub>-Induced Oxidative Stress in <i>Centropomus viridis</i> Brain Cells |
| title_sort | polyphenolic extracts from spent coffee grounds prevent h sub 2 sub o sub 2 sub induced oxidative stress in i centropomus viridis i brain cells |
| topic | antioxidant capacity catalase superoxide dismutase lipid peroxidation white snook |
| url | https://www.mdpi.com/1420-3049/26/20/6195 |
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