Visualization of transcription sites at the electron microscope

In order to localize at EM level the sites of transcription of both pre-mRNA and pre-rRNA, we have detected the DNA/RNA hybrid molecules and m3Gcapped structures by means of specific antibodies after short bromo-uridine (BrU) incorporation. In addition, the sections have been stained by a selective...

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Bibliographic Details
Main Authors: A Trentani, PS Testillano, MC Risueño, M Biggiogera
Format: Article
Language:English
Published: PAGEPress Publications 2009-06-01
Series:European Journal of Histochemistry
Online Access:https://www.ejh.it/index.php/ejh/article/view/827