Automated vitrification of cryo-EM samples with controllable sample thickness using suction and real-time optical inspection
Faster cryo specimen preparation can advance cryo electron microscopy (cryoEM). Here, the authors present a vitrification device with automated sample handling for cryoEM of proteins, suspensions and cells, enabling blot-free sample thinning, dew-point control and characterization of cryo grids prio...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-05-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-022-30562-7 |
| _version_ | 1811254250819289088 |
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| author | Roman I. Koning Hildo Vader Martijn van Nugteren Peter A. Grocutt Wen Yang Ludovic L. R. Renault Abraham J. Koster Arnold C. F. Kamp Michael Schwertner |
| author_facet | Roman I. Koning Hildo Vader Martijn van Nugteren Peter A. Grocutt Wen Yang Ludovic L. R. Renault Abraham J. Koster Arnold C. F. Kamp Michael Schwertner |
| author_sort | Roman I. Koning |
| collection | DOAJ |
| description | Faster cryo specimen preparation can advance cryo electron microscopy (cryoEM). Here, the authors present a vitrification device with automated sample handling for cryoEM of proteins, suspensions and cells, enabling blot-free sample thinning, dew-point control and characterization of cryo grids prior to data acquisition. |
| first_indexed | 2024-04-12T17:03:39Z |
| format | Article |
| id | doaj.art-7d54a3e61dfc4794a06ac42b559224d5 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-04-12T17:03:39Z |
| publishDate | 2022-05-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-7d54a3e61dfc4794a06ac42b559224d52022-12-22T03:24:00ZengNature PortfolioNature Communications2041-17232022-05-0113111010.1038/s41467-022-30562-7Automated vitrification of cryo-EM samples with controllable sample thickness using suction and real-time optical inspectionRoman I. Koning0Hildo Vader1Martijn van Nugteren2Peter A. Grocutt3Wen Yang4Ludovic L. R. Renault5Abraham J. Koster6Arnold C. F. Kamp7Michael Schwertner8Electron Microscopy, Cell and Chemical Biology, Leiden University Medical CenterLinkam Scientific Instruments LtdLinkam Scientific Instruments LtdLinkam Scientific Instruments LtdNeCEN, Institute of Biology Leiden, Leiden UniversityNeCEN, Institute of Biology Leiden, Leiden UniversityElectron Microscopy, Cell and Chemical Biology, Leiden University Medical CenterLinkam Scientific Instruments LtdLinkam Scientific Instruments LtdFaster cryo specimen preparation can advance cryo electron microscopy (cryoEM). Here, the authors present a vitrification device with automated sample handling for cryoEM of proteins, suspensions and cells, enabling blot-free sample thinning, dew-point control and characterization of cryo grids prior to data acquisition.https://doi.org/10.1038/s41467-022-30562-7 |
| spellingShingle | Roman I. Koning Hildo Vader Martijn van Nugteren Peter A. Grocutt Wen Yang Ludovic L. R. Renault Abraham J. Koster Arnold C. F. Kamp Michael Schwertner Automated vitrification of cryo-EM samples with controllable sample thickness using suction and real-time optical inspection Nature Communications |
| title | Automated vitrification of cryo-EM samples with controllable sample thickness using suction and real-time optical inspection |
| title_full | Automated vitrification of cryo-EM samples with controllable sample thickness using suction and real-time optical inspection |
| title_fullStr | Automated vitrification of cryo-EM samples with controllable sample thickness using suction and real-time optical inspection |
| title_full_unstemmed | Automated vitrification of cryo-EM samples with controllable sample thickness using suction and real-time optical inspection |
| title_short | Automated vitrification of cryo-EM samples with controllable sample thickness using suction and real-time optical inspection |
| title_sort | automated vitrification of cryo em samples with controllable sample thickness using suction and real time optical inspection |
| url | https://doi.org/10.1038/s41467-022-30562-7 |
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