Functional traits of Selenium accumulating lactic acid bacteria as a probiont from fermented foods of an Indian tribal community
In the present study, lactic acid bacteria (LAB) from the fermented foods of an Indian tribal community (Irulas) were isolated and screened for Selenium bio-accumulation. A total of 156 isolates were screened, and the isolate Se-M8 from the Khoji (fermented food prepared from the tuber Dioscorea sp....
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| Format: | Article |
| Language: | English |
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Elsevier
2023-06-01
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| Series: | Journal of Agriculture and Food Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666154323001084 |
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| author | Jeyaraj Gnanaprakash Jayamanohar Jabastin Muthu Vijayasarathy Suresh Rajendran Palanisamy Bruntha Devi |
| author_facet | Jeyaraj Gnanaprakash Jayamanohar Jabastin Muthu Vijayasarathy Suresh Rajendran Palanisamy Bruntha Devi |
| author_sort | Jeyaraj Gnanaprakash |
| collection | DOAJ |
| description | In the present study, lactic acid bacteria (LAB) from the fermented foods of an Indian tribal community (Irulas) were isolated and screened for Selenium bio-accumulation. A total of 156 isolates were screened, and the isolate Se-M8 from the Khoji (fermented food prepared from the tuber Dioscorea sp.) accumulated 74.88 mg/L of Selenium. Se-M8 were identified as Enterococcus faecalis by 16S rRNA gene sequencing. This strain was screened for safety assessment, probiotic and antioxidant properties. It showed good tolerance to pH (2 and 3), bile salt (0.3 and 0.6%), transit tolerance, and firm adherence to mucin. It also inhibited the pathogenic biofilm formation by E. coli sturdily. The strain exhibited significant radical scavenging (DPPH֯ and ABTS֯) and strong reducing power activities. Accumulated Selenium particles were extracted, purified and characterised. TEM and SEM analysis revealed the presence of spherical shape particles of 1 μM diameter. This is the first report on the Selenium bio-accumulation by an Enterococcus faecalis strain. Hence, the strain Se-M8 could be used as probiotics with the additional functional role of Selenium supplement to the well-being of human and animal health. Selenium-enriched functional food products could be used as a source of dietary Selenium supplementation for Selenium deficiency disorders and their associated complications. |
| first_indexed | 2024-03-13T10:18:52Z |
| format | Article |
| id | doaj.art-7d659d918f7445f59c300e830ca98dd9 |
| institution | Directory Open Access Journal |
| issn | 2666-1543 |
| language | English |
| last_indexed | 2024-03-13T10:18:52Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Agriculture and Food Research |
| spelling | doaj.art-7d659d918f7445f59c300e830ca98dd92023-05-21T04:35:50ZengElsevierJournal of Agriculture and Food Research2666-15432023-06-0112100601Functional traits of Selenium accumulating lactic acid bacteria as a probiont from fermented foods of an Indian tribal communityJeyaraj Gnanaprakash0Jayamanohar Jabastin1Muthu Vijayasarathy2Suresh Rajendran3Palanisamy Bruntha Devi4Department of Microbial Biotechnology, Bharathiar University, Coimbatore, 641 046, IndiaDepartment of Microbial Biotechnology, Bharathiar University, Coimbatore, 641 046, IndiaDepartment of Microbial Biotechnology, Bharathiar University, Coimbatore, 641 046, IndiaDepartment of Microbial Biotechnology, Bharathiar University, Coimbatore, 641 046, IndiaCorresponding author.; Department of Microbial Biotechnology, Bharathiar University, Coimbatore, 641 046, IndiaIn the present study, lactic acid bacteria (LAB) from the fermented foods of an Indian tribal community (Irulas) were isolated and screened for Selenium bio-accumulation. A total of 156 isolates were screened, and the isolate Se-M8 from the Khoji (fermented food prepared from the tuber Dioscorea sp.) accumulated 74.88 mg/L of Selenium. Se-M8 were identified as Enterococcus faecalis by 16S rRNA gene sequencing. This strain was screened for safety assessment, probiotic and antioxidant properties. It showed good tolerance to pH (2 and 3), bile salt (0.3 and 0.6%), transit tolerance, and firm adherence to mucin. It also inhibited the pathogenic biofilm formation by E. coli sturdily. The strain exhibited significant radical scavenging (DPPH֯ and ABTS֯) and strong reducing power activities. Accumulated Selenium particles were extracted, purified and characterised. TEM and SEM analysis revealed the presence of spherical shape particles of 1 μM diameter. This is the first report on the Selenium bio-accumulation by an Enterococcus faecalis strain. Hence, the strain Se-M8 could be used as probiotics with the additional functional role of Selenium supplement to the well-being of human and animal health. Selenium-enriched functional food products could be used as a source of dietary Selenium supplementation for Selenium deficiency disorders and their associated complications.http://www.sciencedirect.com/science/article/pii/S2666154323001084Fermented foodsLactic acid bacteriaEnterococcus faecalisSelenium accumulationProbioticsFunctional traits |
| spellingShingle | Jeyaraj Gnanaprakash Jayamanohar Jabastin Muthu Vijayasarathy Suresh Rajendran Palanisamy Bruntha Devi Functional traits of Selenium accumulating lactic acid bacteria as a probiont from fermented foods of an Indian tribal community Journal of Agriculture and Food Research Fermented foods Lactic acid bacteria Enterococcus faecalis Selenium accumulation Probiotics Functional traits |
| title | Functional traits of Selenium accumulating lactic acid bacteria as a probiont from fermented foods of an Indian tribal community |
| title_full | Functional traits of Selenium accumulating lactic acid bacteria as a probiont from fermented foods of an Indian tribal community |
| title_fullStr | Functional traits of Selenium accumulating lactic acid bacteria as a probiont from fermented foods of an Indian tribal community |
| title_full_unstemmed | Functional traits of Selenium accumulating lactic acid bacteria as a probiont from fermented foods of an Indian tribal community |
| title_short | Functional traits of Selenium accumulating lactic acid bacteria as a probiont from fermented foods of an Indian tribal community |
| title_sort | functional traits of selenium accumulating lactic acid bacteria as a probiont from fermented foods of an indian tribal community |
| topic | Fermented foods Lactic acid bacteria Enterococcus faecalis Selenium accumulation Probiotics Functional traits |
| url | http://www.sciencedirect.com/science/article/pii/S2666154323001084 |
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