Editing efficiencies with Cas9 orthologs, Cas12a endonucleases, and temperature in rice

The advent of CRISPR-Cas technology has made it the genome editing tool of choice in all kingdoms of life, including plants, which can have large, highly duplicated genomes. As a result, finding adequate target sequences that meet the specificities of a given Cas nuclease on any gene of interest rem...

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Bibliographic Details
Main Authors: Eudald Illa-Berenguer, Peter R. LaFayette, Wayne A. Parrott
Format: Article
Language:English
Published: Frontiers Media S.A. 2023-03-01
Series:Frontiers in Genome Editing
Subjects:
Online Access:https://www.frontiersin.org/articles/10.3389/fgeed.2023.1074641/full