Editing efficiencies with Cas9 orthologs, Cas12a endonucleases, and temperature in rice
The advent of CRISPR-Cas technology has made it the genome editing tool of choice in all kingdoms of life, including plants, which can have large, highly duplicated genomes. As a result, finding adequate target sequences that meet the specificities of a given Cas nuclease on any gene of interest rem...
Main Authors: | , , |
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Format: | Article |
Language: | English |
Published: |
Frontiers Media S.A.
2023-03-01
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Series: | Frontiers in Genome Editing |
Subjects: | |
Online Access: | https://www.frontiersin.org/articles/10.3389/fgeed.2023.1074641/full |