Comparative effects of two in situ hybridization methods for the pinewood nematode (Bursaphelenchus xylophilus)

The gene localization technique of Bursaphelenchus xylophilus (pinewood nematode, PWN) is used for study gene expression in PWNs. Two in situ hybridization methods, namely, whole-mount in situ hybridization and the cut-off method are used widely. To compare the effects of these two in situ hybridiza...

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Bibliographic Details
Main Authors: Chunyu Wang, Junhao Zhuge, Siqi Tang, Xiang Zhou, Lifeng Zhou, Kai Guo
Format: Article
Language:English
Published: Frontiers Media S.A. 2023-11-01
Series:Frontiers in Microbiology
Subjects:
Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2023.1234895/full