Site-specific analysis of ribosomal 2′O-methylation by quantitative reverse transcription PCR under low deoxynucleotide triphosphate concentrations
Ribose 2′O-methylation (Nm, ribomethylation) is the most abundant RNA modification present in rRNA. It has been shown that alterations in ribosomal 2′O-methylation at individual Nm sites likely reflect regulated cellular processes. Although several analytical approaches for Nm detection and profilin...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Future Science Ltd
2023-05-01
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| Series: | BioTechniques |
| Subjects: | |
| Online Access: | https://www.future-science.com/doi/10.2144/btn-2022-0122 |