Magnetic, Microplate-Format Plasmid Isolation Protocol for High-Yield, Sequencing-Grade DNA
We have developed a rapid, microplateformat plasmid isolation procedure to purify sequencing-grade DNA templates for high-throughput DNA sequencing operations. A modified lysozyme/boiling method is used to produce a plasmid-containing supernatant that is then purified by iron bead capture. After bin...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2000-10-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/00294st05 |