Dual-Channel Stopped-Flow Apparatus for Simultaneous Fluorescence, Anisotropy, and FRET Kinetic Data Acquisition for Binary and Ternary Biological Complexes

Dual-Channel Stopped-Flow Apparatus for Simultaneous Fluorescence, Anisotropy, and FRET Kinetic Data Acquisition for Binary and Ternary Biological Complexes

The Stopped-Flow apparatus (SF) tracks molecular events by mixing the reactants in sub-millisecond regimes. The reaction of intrinsically or extrinsically labeled biomolecules can be monitored by recording the fluorescence, <i>F</i>(<i>t</i>), anisotropy, <i>r</i>...

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Bibliographic Details
Main Authors: Roberto F. Delgadillo, Katie A. Carnes, Nestor Valles-Villarreal, Omar Olmos, Kathia Zaleta-Rivera, Lawrence J. Parkhurst
Format: Article
Language:English
Published: MDPI AG 2020-11-01
Series:Biosensors
Subjects:
stopped-flow
kinetics
fluorescence
anisotropy
polarizers
magic angle
Online Access:https://www.mdpi.com/2079-6374/10/11/180

Internet

https://www.mdpi.com/2079-6374/10/11/180

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