Characterization of Biosensors Based on Recombinant Glutamate Oxidase: Comparison of Crosslinking Agents in Terms of Enzyme Loading and Efficiency Parameters

Characterization of Biosensors Based on Recombinant Glutamate Oxidase: Comparison of Crosslinking Agents in Terms of Enzyme Loading and Efficiency Parameters

Amperometric l-glutamate (Glu) biosensors, based on both wild-type and a recombinant form of l-glutamate oxidase (GluOx), were designed and characterized in terms of enzyme-kinetic, sensitivity and stability parameters in attempts to fabricate a real-time Glu monitoring device suitable for future lo...

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Bibliographic Details
Main Authors: Rochelle Ford, Susan J. Quinn, Robert D. O’Neill
Format: Article
Language:English
Published: MDPI AG 2016-09-01
Series:Sensors
Subjects:
glutamate biosensor stability
biomedical applications
amperometry
surface enzyme loading and affinity
permselective polymer
poly(ortho-phenylenediamine)
Online Access:http://www.mdpi.com/1424-8220/16/10/1565

Internet

http://www.mdpi.com/1424-8220/16/10/1565

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