In vitro quantification of pigment production and transfer in 2D co-cultures and 3D skin organotypic

In vitro quantification of pigment production and transfer in 2D co-cultures and 3D skin organotypic

Summary: Here, we present a protocol to set up and study 2D keratinocyte-melanocyte co-cultures and 3D full-thickness human skin equivalents. We describe steps for culturing of keratinocyte and melanocyte lines and the establishment of both 2D and 3D co-cultures. The cultures are utilized to measure...

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Bibliographic Details
Main Authors: Rachel Phua, Tracy Zhijun Tien, Gracia Goh, Nikolaos Pantelireis, Kenice Siew Hoon Sim, Jamien Lim, Carlos Clavel
Format: Article
Language:English
Published: Elsevier 2023-06-01
Series:STAR Protocols
Subjects:
Cell culture
Cell isolation
Developmental biology
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723003015

Internet

http://www.sciencedirect.com/science/article/pii/S2666166723003015

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