Robustness of biomarker determination in breast cancer by RT-qPCR: impact of tumor cell content, DCIS and non-neoplastic breast tissue

Robustness of biomarker determination in breast cancer by RT-qPCR: impact of tumor cell content, DCIS and non-neoplastic breast tissue

Abstract Background Tissue heterogeneity in formalin-fixed paraffin-embedded (FFPE) breast cancer specimens may affect the accuracy of reverse transcription quantitative real-time PCR (RT-qPCR). Herein, we tested the impact of tissue heterogeneity of breast cancer specimen on the RT-qPCR-based gene...

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Bibliographic Details
Main Authors: Kerstin Hartmann, Kornelia Schlombs, Mark Laible, Claudia Gürtler, Marcus Schmidt, Ugur Sahin, Hans-Anton Lehr
Format: Article
Language:English
Published: BMC 2018-10-01
Series:Diagnostic Pathology
Subjects:
Breast cancer
MammaTyper
RT-qPCR
DCIS
Tumor cell content
Non-neoplastic tissue
Online Access:http://link.springer.com/article/10.1186/s13000-018-0760-6

Internet

http://link.springer.com/article/10.1186/s13000-018-0760-6

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