Structural basis for two metal-ion catalysis of DNA cleavage by Cas12i2
Cas12i, class 2 type V CRISPR-Cas system protein, uses a single RuvC domain for cleavage of both strands of target DNA. Structures of Cas12i2–crRNA–DNA complexes not only provide insight into the mechanism of DNA recognition and cleavage by Cas12i2, but also the DNA cleavage mechanism by RuvC-contai...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2020-10-01
|
| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-020-19072-6 |