Structural basis for two metal-ion catalysis of DNA cleavage by Cas12i2
Cas12i, class 2 type V CRISPR-Cas system protein, uses a single RuvC domain for cleavage of both strands of target DNA. Structures of Cas12i2–crRNA–DNA complexes not only provide insight into the mechanism of DNA recognition and cleavage by Cas12i2, but also the DNA cleavage mechanism by RuvC-contai...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2020-10-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-020-19072-6 |
| _version_ | 1818742236133720064 |
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| author | Xue Huang Wei Sun Zhi Cheng Minxuan Chen Xueyan Li Jiuyu Wang Gang Sheng Weimin Gong Yanli Wang |
| author_facet | Xue Huang Wei Sun Zhi Cheng Minxuan Chen Xueyan Li Jiuyu Wang Gang Sheng Weimin Gong Yanli Wang |
| author_sort | Xue Huang |
| collection | DOAJ |
| description | Cas12i, class 2 type V CRISPR-Cas system protein, uses a single RuvC domain for cleavage of both strands of target DNA. Structures of Cas12i2–crRNA–DNA complexes not only provide insight into the mechanism of DNA recognition and cleavage by Cas12i2, but also the DNA cleavage mechanism by RuvC-containing Cas proteins. |
| first_indexed | 2024-12-18T02:09:18Z |
| format | Article |
| id | doaj.art-7e8c055909804b818ce2b9ea4c5129d9 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-12-18T02:09:18Z |
| publishDate | 2020-10-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-7e8c055909804b818ce2b9ea4c5129d92022-12-21T21:24:31ZengNature PortfolioNature Communications2041-17232020-10-0111111410.1038/s41467-020-19072-6Structural basis for two metal-ion catalysis of DNA cleavage by Cas12i2Xue Huang0Wei Sun1Zhi Cheng2Minxuan Chen3Xueyan Li4Jiuyu Wang5Gang Sheng6Weimin Gong7Yanli Wang8Hefei National Laboratory for Physical Sciences at the Microscales, University of Science and Technology of ChinaNational Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of SciencesNational Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of SciencesNational Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of SciencesNational Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of SciencesNational Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of SciencesKey Laboratory of RNA Biology, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of SciencesHefei National Laboratory for Physical Sciences at the Microscales, University of Science and Technology of ChinaNational Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of SciencesCas12i, class 2 type V CRISPR-Cas system protein, uses a single RuvC domain for cleavage of both strands of target DNA. Structures of Cas12i2–crRNA–DNA complexes not only provide insight into the mechanism of DNA recognition and cleavage by Cas12i2, but also the DNA cleavage mechanism by RuvC-containing Cas proteins.https://doi.org/10.1038/s41467-020-19072-6 |
| spellingShingle | Xue Huang Wei Sun Zhi Cheng Minxuan Chen Xueyan Li Jiuyu Wang Gang Sheng Weimin Gong Yanli Wang Structural basis for two metal-ion catalysis of DNA cleavage by Cas12i2 Nature Communications |
| title | Structural basis for two metal-ion catalysis of DNA cleavage by Cas12i2 |
| title_full | Structural basis for two metal-ion catalysis of DNA cleavage by Cas12i2 |
| title_fullStr | Structural basis for two metal-ion catalysis of DNA cleavage by Cas12i2 |
| title_full_unstemmed | Structural basis for two metal-ion catalysis of DNA cleavage by Cas12i2 |
| title_short | Structural basis for two metal-ion catalysis of DNA cleavage by Cas12i2 |
| title_sort | structural basis for two metal ion catalysis of dna cleavage by cas12i2 |
| url | https://doi.org/10.1038/s41467-020-19072-6 |
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