Combination of CENP-B Box Positive and Negative Synthetic Alpha Satellite Repeats Improves De Novo Human Artificial Chromosome Formation

Combination of CENP-B Box Positive and Negative Synthetic Alpha Satellite Repeats Improves De Novo Human Artificial Chromosome Formation

Human artificial chromosomes (HACs) can be formed de novo by introducing large (>30 kb) centromeric sequences consisting of highly repeated 171-bp alpha satellite (alphoid) DNA into HT1080 cells. However, only a subset of transformed cells successfully establishes HACs. CENP-A chromatin and heter...

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Bibliographic Details
Main Authors: Koei Okazaki, Megumi Nakano, Jun-ichirou Ohzeki, Koichiro Otake, Kazuto Kugou, Vladimir Larionov, William C. Earnshaw, Hiroshi Masumoto
Format: Article
Language:English
Published: MDPI AG 2022-04-01
Series:Cells
Subjects:
HAC
chromatin
alphoid
CENP-B
histone
centromere
Online Access:https://www.mdpi.com/2073-4409/11/9/1378

Internet

https://www.mdpi.com/2073-4409/11/9/1378

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