The Humanised NPY-mRFP RBL Reporter Cell Line Is a Fast and Inexpensive Tool for Detection of Allergen-Specific IgE in Human Sera

The Humanised NPY-mRFP RBL Reporter Cell Line Is a Fast and Inexpensive Tool for Detection of Allergen-Specific IgE in Human Sera

Rat basophilic leukaemia (RBL) cells have been used for decades as a model of high-affinity Immunoglobulin E (IgE) receptor (FcεRI) signalling. Here, we describe the generation and use of huNPY-mRFP, a new humanised fluorescent IgE reporter cell line. Fusion of Neuropeptide Y (NPY) with monomeric re...

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Bibliographic Details
Main Authors: Prema S. Prakash, Nafal J. S. Barwary, Michael H. W. Weber, Daniel Wan, Iván Conejeros, Bernardo Pereira Moreira, Waleed S. Alharbi, Jaap J. van Hellemond, Jude Akinwale, Franco H. Falcone
Format: Article
Language:English
Published: MDPI AG 2022-08-01
Series:Diagnostics
Subjects:
NPY-mRFP
RBL
reporter system
IgE
<i>Echinococcus granulosus</i>
EgEF-1β/δ
Online Access:https://www.mdpi.com/2075-4418/12/9/2063

Internet

https://www.mdpi.com/2075-4418/12/9/2063

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