Effect OF m-trifluoromethyl- attachment on the glutathione peroxidase mimicry and antioxidant actions of diphenyl diselenide: Essential thiols of electrogenic sodium pump as a mechanistic component
m-Ditrifluoromethyl-diphenyl diselenide [DFDD (m-CF3-C6H4Se)2] is a disubstituted diaryl analog of diphenyl diselenide [DPDS (C6H5Se)2] in which a hydrogen atom on each aromatic ring is replaced by trifluoromethyl group (-CF3). Herein, we investigated the effect of the -CF3 group introduction on the...
| Những tác giả chính: | , , , , , |
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| Định dạng: | Bài viết |
| Ngôn ngữ: | English |
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Elsevier
2024-04-01
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| Loạt: | Advances in Redox Research |
| Những chủ đề: | |
| Truy cập trực tuyến: | http://www.sciencedirect.com/science/article/pii/S2667137923000322 |
| _version_ | 1827316927474696192 |
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| author | Ebenezer Morayo Ale Steve Osagie Asuelimen Victoria Ifeoluwa Ayo Mgbede Joy Timothy Isaac John Umaru Ebenezer Kayode Toluwalase |
| author_facet | Ebenezer Morayo Ale Steve Osagie Asuelimen Victoria Ifeoluwa Ayo Mgbede Joy Timothy Isaac John Umaru Ebenezer Kayode Toluwalase |
| author_sort | Ebenezer Morayo Ale |
| collection | DOAJ |
| description | m-Ditrifluoromethyl-diphenyl diselenide [DFDD (m-CF3-C6H4Se)2] is a disubstituted diaryl analog of diphenyl diselenide [DPDS (C6H5Se)2] in which a hydrogen atom on each aromatic ring is replaced by trifluoromethyl group (-CF3). Herein, we investigated the effect of the -CF3 group introduction on the GPx mimetic and antioxidant properties of DPDS. Animals were euthanized, brains were removed, and used for lipid peroxidation, cerebral sodium pump activity and thiols assays in vitro. Results showed that DFDD utilizes exogenous thiols [dithiol treitol (DTT), cysteine (Cys) and glutathione (GSH)] to reduce hydroperoxides. Furthermore, DFDD only protected against deoxyribose degradation in the presence of DTT. DFDD also exerted marked (p< 0.05) inhibitory effect on Fe2+or H2O2 or fenton reaction-induced lipid peroxidation in rat cerebral tissue homogenate. In addition, DFDD simultaneously (p< 0.05) inhibited pump activity and lipid peroxidation in cerebral tissue homogenate assaulted with prooxidants with proportionate depletion of thiol in the reaction system. This assay was repeated in the presence of DTT or Cys-or GSH and results revealed that enzyme's activity was not inhibited indicating that DFDD switched from enzymes's thiols to the oxidation of medium's thiols. It is rational to conclude that the introduction of -CF3 group to the aromatic rings of DFDD does not abolish its GPx mimetic and antioxidant properties and these still rely on thiols of cerebral electrogenic sodium pump. DFDD could be a suitable candidate for relative pharmacological effect and weak toxicity consequent to its possession of high electron withdrawing group. However, further research is needed in this regard. |
| first_indexed | 2024-03-08T14:35:18Z |
| format | Article |
| id | doaj.art-7ef34c00929f4e379ff9ffecac58d41b |
| institution | Directory Open Access Journal |
| issn | 2667-1379 |
| language | English |
| last_indexed | 2024-04-24T23:22:50Z |
| publishDate | 2024-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Advances in Redox Research |
| spelling | doaj.art-7ef34c00929f4e379ff9ffecac58d41b2024-03-16T05:09:54ZengElsevierAdvances in Redox Research2667-13792024-04-0110100092Effect OF m-trifluoromethyl- attachment on the glutathione peroxidase mimicry and antioxidant actions of diphenyl diselenide: Essential thiols of electrogenic sodium pump as a mechanistic componentEbenezer Morayo Ale0Steve Osagie Asuelimen1Victoria Ifeoluwa Ayo2Mgbede Joy Timothy3Isaac John Umaru4Ebenezer Kayode Toluwalase5Department of Biochemistry, Faculty of Pure and Applied Sciences, Federal University Wukari, Taraba State, Nigeria; Corresponding author.Department of Biochemistry, Faculty of Pure and Applied Sciences, Federal University Wukari, Taraba State, NigeriaDepartment of Biochemistry, Faculty of Pure and Applied Sciences, Federal University Wukari, Taraba State, NigeriaDepartment of Biochemistry, Faculty of Pure and Applied Sciences, Federal University Wukari, Taraba State, NigeriaDepartment of Biochemistry, Faculty of Pure and Applied Sciences, Federal University Wukari, Taraba State, NigeriaDepartment of Biochemistry, School of Sciences, Federal University of Technology, Akure, Ondo State, Nigeriam-Ditrifluoromethyl-diphenyl diselenide [DFDD (m-CF3-C6H4Se)2] is a disubstituted diaryl analog of diphenyl diselenide [DPDS (C6H5Se)2] in which a hydrogen atom on each aromatic ring is replaced by trifluoromethyl group (-CF3). Herein, we investigated the effect of the -CF3 group introduction on the GPx mimetic and antioxidant properties of DPDS. Animals were euthanized, brains were removed, and used for lipid peroxidation, cerebral sodium pump activity and thiols assays in vitro. Results showed that DFDD utilizes exogenous thiols [dithiol treitol (DTT), cysteine (Cys) and glutathione (GSH)] to reduce hydroperoxides. Furthermore, DFDD only protected against deoxyribose degradation in the presence of DTT. DFDD also exerted marked (p< 0.05) inhibitory effect on Fe2+or H2O2 or fenton reaction-induced lipid peroxidation in rat cerebral tissue homogenate. In addition, DFDD simultaneously (p< 0.05) inhibited pump activity and lipid peroxidation in cerebral tissue homogenate assaulted with prooxidants with proportionate depletion of thiol in the reaction system. This assay was repeated in the presence of DTT or Cys-or GSH and results revealed that enzyme's activity was not inhibited indicating that DFDD switched from enzymes's thiols to the oxidation of medium's thiols. It is rational to conclude that the introduction of -CF3 group to the aromatic rings of DFDD does not abolish its GPx mimetic and antioxidant properties and these still rely on thiols of cerebral electrogenic sodium pump. DFDD could be a suitable candidate for relative pharmacological effect and weak toxicity consequent to its possession of high electron withdrawing group. However, further research is needed in this regard.http://www.sciencedirect.com/science/article/pii/S2667137923000322OrganoseleniumsDiphenyl diselenidem-trifluoromethyl-diphenyl diselenideGlutathione peroxidaseAntioxidantThiols |
| spellingShingle | Ebenezer Morayo Ale Steve Osagie Asuelimen Victoria Ifeoluwa Ayo Mgbede Joy Timothy Isaac John Umaru Ebenezer Kayode Toluwalase Effect OF m-trifluoromethyl- attachment on the glutathione peroxidase mimicry and antioxidant actions of diphenyl diselenide: Essential thiols of electrogenic sodium pump as a mechanistic component Advances in Redox Research Organoseleniums Diphenyl diselenide m-trifluoromethyl-diphenyl diselenide Glutathione peroxidase Antioxidant Thiols |
| title | Effect OF m-trifluoromethyl- attachment on the glutathione peroxidase mimicry and antioxidant actions of diphenyl diselenide: Essential thiols of electrogenic sodium pump as a mechanistic component |
| title_full | Effect OF m-trifluoromethyl- attachment on the glutathione peroxidase mimicry and antioxidant actions of diphenyl diselenide: Essential thiols of electrogenic sodium pump as a mechanistic component |
| title_fullStr | Effect OF m-trifluoromethyl- attachment on the glutathione peroxidase mimicry and antioxidant actions of diphenyl diselenide: Essential thiols of electrogenic sodium pump as a mechanistic component |
| title_full_unstemmed | Effect OF m-trifluoromethyl- attachment on the glutathione peroxidase mimicry and antioxidant actions of diphenyl diselenide: Essential thiols of electrogenic sodium pump as a mechanistic component |
| title_short | Effect OF m-trifluoromethyl- attachment on the glutathione peroxidase mimicry and antioxidant actions of diphenyl diselenide: Essential thiols of electrogenic sodium pump as a mechanistic component |
| title_sort | effect of m trifluoromethyl attachment on the glutathione peroxidase mimicry and antioxidant actions of diphenyl diselenide essential thiols of electrogenic sodium pump as a mechanistic component |
| topic | Organoseleniums Diphenyl diselenide m-trifluoromethyl-diphenyl diselenide Glutathione peroxidase Antioxidant Thiols |
| url | http://www.sciencedirect.com/science/article/pii/S2667137923000322 |
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