FAST, a method based on split-GFP for the detection in solution of proteins synthesized in cell-free expression systems

FAST, a method based on split-GFP for the detection in solution of proteins synthesized in cell-free expression systems

Abstract Cell-free protein synthesis (CFPS) systems offer a versatile platform for a wide range of applications. However, the traditional methods for detecting proteins synthesized in CFPS, such as radioactive labeling, fluorescent tagging, or electrophoretic separation, may be impractical, due to e...

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Bibliographic Details
Main Authors: Thuy Duong Pham, Chiara Poletti, Therese Manuela Nloh Tientcheu, Massimiliano Cuccioloni, Roberto Spurio, Attilio Fabbretti, Pohl Milon, Anna Maria Giuliodori
Format: Article
Language:English
Published: Nature Portfolio 2024-04-01
Series:Scientific Reports
Subjects:
Split-GFP
Protein synthesis
CFPS
Fluorescence
CspA
Online Access:https://doi.org/10.1038/s41598-024-58588-5

Internet

https://doi.org/10.1038/s41598-024-58588-5

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