Unraveling the salt tolerance of Phi29 DNA polymerase using compartmentalized self-replication and microfluidics platform

Unraveling the salt tolerance of Phi29 DNA polymerase using compartmentalized self-replication and microfluidics platform

In Phi29-α–hemolysin (α-HL) nanopore sequencing systems, a strong electrochemical signal is dependent on a high concentration of salt. However, high salt concentrations adversely affect polymerase activity. Sequencing by synthesis (SBS) requires the use of phi29 polymerase without exonuclease activi...

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Bibliographic Details
Main Authors: Yaping Sun, Danny Hsu Ko, Jie Gao, Kang Fu, Yaping Gao, Qiwen Zhang, Salem Baldi, Tao Hong, Igor Ivanov, Yun He, Hui Tian
Format: Article
Language:English
Published: Frontiers Media S.A. 2023-11-01
Series:Frontiers in Microbiology
Subjects:
compartmentalized self-replication (CSR)
sequencing by synthesis (SBS)
salt tolerance polymerase
droplet-based microfluidics
conservative amino acids
Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2023.1267196/full

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https://www.frontiersin.org/articles/10.3389/fmicb.2023.1267196/full

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