High-throughput Characterization of HIV-1 Reservoir Reactivation Using a Single-Cell-in-Droplet PCR Assay

High-throughput Characterization of HIV-1 Reservoir Reactivation Using a Single-Cell-in-Droplet PCR Assay

Reactivation of latent viral reservoirs is on the forefront of HIV-1 eradication research. However, it is unknown if latency reversing agents (LRAs) increase the level of viral transcription from cells producing HIV RNA or harboring transcriptionally-inactive (latent) infection. We therefore develop...

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Bibliographic Details
Main Authors: Robert W. Yucha, Kristen S. Hobbs, Emily Hanhauser, Louise E. Hogan, Wildaliz Nieves, Mehmet O. Ozen, Fatih Inci, Vanessa York, Erica A. Gibson, Cassandra Thanh, Hadi Shafiee, Rami El Assal, Maja Kiselinova, Yvonne P. Robles, Helen Bae, Kaitlyn S. Leadabrand, ShuQi Wang, Steven G. Deeks, Daniel R. Kuritzkes, Utkan Demirci, Timothy J. Henrich
Format: Article
Language:English
Published: Elsevier 2017-06-01
Series:EBioMedicine
Subjects:
Human Immunodeficiency Virus (HIV)
Single cell quantification
Digital PCR
HIV reservoirs
HIV reactivation
Histone deacetylase inhibitors
Online Access:http://www.sciencedirect.com/science/article/pii/S2352396417301974

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http://www.sciencedirect.com/science/article/pii/S2352396417301974

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