Development of an in vitro method for activation of X-succinate synthases for fumarate hydroalkylation

Summary: Anaerobic microbial degradation of hydrocarbons is often initiated through addition of the hydrocarbon to fumarate by enzymes known as X-succinate synthases (XSSs). XSSs use a glycyl radical cofactor, which is installed by an activating enzyme (XSS-AE), to catalyze this carbon-carbon coupli...

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Bibliographic Details
Main Authors: Mary C. Andorfer, Devin T. King-Roberts, Christa N. Imrich, Balyn G. Brotheridge, Catherine L. Drennan
Format: Article
Language:English
Published: Elsevier 2023-06-01
Series:iScience
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2589004223009793