Protective Effect of <i>Lycium ruthenicum</i> Polyphenols on Oxidative Stress against Acrylamide Induced Liver Injury in Rats
Acrylamide (ACR) is formed during tobacco and carbohydrate-rich food heating and is widely applied in many industries, with a range of toxic effects. The antioxidant properties of <i>Lycium ruthenicum</i> polyphenols (LRP) have been established before. This study aimed to research the pr...
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MDPI AG
2022-06-01
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| author | Hua Gao Yanzhong Xue Lingyu Wu Jinghong Huo Yufei Pang Jingxin Chen Qinghan Gao |
| author_facet | Hua Gao Yanzhong Xue Lingyu Wu Jinghong Huo Yufei Pang Jingxin Chen Qinghan Gao |
| author_sort | Hua Gao |
| collection | DOAJ |
| description | Acrylamide (ACR) is formed during tobacco and carbohydrate-rich food heating and is widely applied in many industries, with a range of toxic effects. The antioxidant properties of <i>Lycium ruthenicum</i> polyphenols (LRP) have been established before. This study aimed to research the protective effect of LRP against ACR-induced liver injury in SD rats. Rats were divided into six groups: Control, ACR (40 mg/kg/day, i.g.), LRP (50, 100, and 200 mg/kg/day, i.g.) plus ACR, and LRP groups. After 19 days, we evaluated oxidative status and mitochondrial functions in the rat’s liver. The results showed that glutathione (GSH) and superoxide dismutase (SOD) levels increased after LRP pretreatment. In contrast, each intervention group reduced reactive oxygen species (ROS) and malondialdehyde (MDA) levels compared to the ACR group. Meanwhile, alanine aminotransferase (ALT), aspartate aminotransferase (AST), liver mitochondrial ATPase activity, mRNA expression of mitochondrial complex I, III, and expression of nuclear factor-erythroid 2-related factor 2 (Nrf2) and its downstream proteins were all increased. This study suggested that LRP could reduce ACR-induced liver injury through potent antioxidant activity. LRP is recommended as oxidative stress reliever against hepatotoxicity. |
| first_indexed | 2024-03-09T04:01:35Z |
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| id | doaj.art-7f18fcc30c224eb1a7e748d0f59eb11a |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T04:01:35Z |
| publishDate | 2022-06-01 |
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| record_format | Article |
| series | Molecules |
| spelling | doaj.art-7f18fcc30c224eb1a7e748d0f59eb11a2023-12-03T14:13:20ZengMDPI AGMolecules1420-30492022-06-012713410010.3390/molecules27134100Protective Effect of <i>Lycium ruthenicum</i> Polyphenols on Oxidative Stress against Acrylamide Induced Liver Injury in RatsHua Gao0Yanzhong Xue1Lingyu Wu2Jinghong Huo3Yufei Pang4Jingxin Chen5Qinghan Gao6Department of Pharmacy, General Hospital of Ningxia Medical University, Yinchuan 750004, ChinaSchool of Public Health and Management, Ningxia Medical University, Yinchuan 750004, ChinaSchool of Public Health and Management, Ningxia Medical University, Yinchuan 750004, ChinaSchool of Public Health and Management, Ningxia Medical University, Yinchuan 750004, ChinaSchool of Public Health and Management, Ningxia Medical University, Yinchuan 750004, ChinaSchool of Public Health and Management, Ningxia Medical University, Yinchuan 750004, ChinaSchool of Public Health and Management, Ningxia Medical University, Yinchuan 750004, ChinaAcrylamide (ACR) is formed during tobacco and carbohydrate-rich food heating and is widely applied in many industries, with a range of toxic effects. The antioxidant properties of <i>Lycium ruthenicum</i> polyphenols (LRP) have been established before. This study aimed to research the protective effect of LRP against ACR-induced liver injury in SD rats. Rats were divided into six groups: Control, ACR (40 mg/kg/day, i.g.), LRP (50, 100, and 200 mg/kg/day, i.g.) plus ACR, and LRP groups. After 19 days, we evaluated oxidative status and mitochondrial functions in the rat’s liver. The results showed that glutathione (GSH) and superoxide dismutase (SOD) levels increased after LRP pretreatment. In contrast, each intervention group reduced reactive oxygen species (ROS) and malondialdehyde (MDA) levels compared to the ACR group. Meanwhile, alanine aminotransferase (ALT), aspartate aminotransferase (AST), liver mitochondrial ATPase activity, mRNA expression of mitochondrial complex I, III, and expression of nuclear factor-erythroid 2-related factor 2 (Nrf2) and its downstream proteins were all increased. This study suggested that LRP could reduce ACR-induced liver injury through potent antioxidant activity. LRP is recommended as oxidative stress reliever against hepatotoxicity.https://www.mdpi.com/1420-3049/27/13/4100acrylamidehepatotoxicity<i>Lycium ruthenicum</i>mitochondrial functionoxidative stress |
| spellingShingle | Hua Gao Yanzhong Xue Lingyu Wu Jinghong Huo Yufei Pang Jingxin Chen Qinghan Gao Protective Effect of <i>Lycium ruthenicum</i> Polyphenols on Oxidative Stress against Acrylamide Induced Liver Injury in Rats Molecules acrylamide hepatotoxicity <i>Lycium ruthenicum</i> mitochondrial function oxidative stress |
| title | Protective Effect of <i>Lycium ruthenicum</i> Polyphenols on Oxidative Stress against Acrylamide Induced Liver Injury in Rats |
| title_full | Protective Effect of <i>Lycium ruthenicum</i> Polyphenols on Oxidative Stress against Acrylamide Induced Liver Injury in Rats |
| title_fullStr | Protective Effect of <i>Lycium ruthenicum</i> Polyphenols on Oxidative Stress against Acrylamide Induced Liver Injury in Rats |
| title_full_unstemmed | Protective Effect of <i>Lycium ruthenicum</i> Polyphenols on Oxidative Stress against Acrylamide Induced Liver Injury in Rats |
| title_short | Protective Effect of <i>Lycium ruthenicum</i> Polyphenols on Oxidative Stress against Acrylamide Induced Liver Injury in Rats |
| title_sort | protective effect of i lycium ruthenicum i polyphenols on oxidative stress against acrylamide induced liver injury in rats |
| topic | acrylamide hepatotoxicity <i>Lycium ruthenicum</i> mitochondrial function oxidative stress |
| url | https://www.mdpi.com/1420-3049/27/13/4100 |
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