Fenugreek seed ethanolic extract inhibited formation of advanced glycation end products via scavenging reactive carbonyl intermediates
Senescence is a natural phenomenon of growing old. It accelerates under certain conditions like diabetes mellitus resulting in early decline of bodily functions, which can be avoided by many claimed functional foods. The present study aims to investigate the anti-aging ability of Fenugreek seeds (Tr...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-06-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844023040732 |
| _version_ | 1797807183589539840 |
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| author | Laila Anwar Syed Abid Ali Sana Khan Mir Muhammad Uzairullah Nazish Mustafa Urooj Anwer Ali Faheema Siddiqui Huma Aslam Bhatti Syed Jawad Rehmani Ghulam Abbas |
| author_facet | Laila Anwar Syed Abid Ali Sana Khan Mir Muhammad Uzairullah Nazish Mustafa Urooj Anwer Ali Faheema Siddiqui Huma Aslam Bhatti Syed Jawad Rehmani Ghulam Abbas |
| author_sort | Laila Anwar |
| collection | DOAJ |
| description | Senescence is a natural phenomenon of growing old. It accelerates under certain conditions like diabetes mellitus resulting in early decline of bodily functions, which can be avoided by many claimed functional foods. The present study aims to investigate the anti-aging ability of Fenugreek seeds (Trigonellafoenum-graecum); a common ingredient of Indo-Pak cuisines. Briefly, the Fenugreek seeds extract (FgSE) in concentrationsof0.1, 0.5 and 1 mg/ml inhibited the formation of Advanced Glycation End products (AGEs) and fructosamine adducts in Bovine serum albumin (BSA)/fructose model in vitro. The BSA conformational analysis via Circular Dichorism and Congo red assays showed that it preserves secondary structure of BSA in aforementioned model. Although mechanistic studies revealed insignificant lysine blocking ability of Fenugreek by OPA assay, however carbonyl entrapping was found to be 24%, 34% and 42% at 0.1, 0.5 and 1 mg/ml, respectively. In vivo model of High Fructose diet (HFD) induced glycation, FgSE treatment in doses of 10, 25 & 50 mg/kg markedly improved Escape latency (p < 0.01) and preserved cognition in Morris Water Maze. Our data further exhibits significant decrease of CML (Nε-carboxymethyl lysine) levels in serum and hippocampus byFgSE treatment in comparison with HFD group. Therefore, we deduced that FgSE prevents glycation-induced memory decline via entrapping the reactive carbonyl intermediates, formed during production of AGEs. Hence, as a promising functional food it slows down the harmful process of glycation and aging associated morbidities. |
| first_indexed | 2024-03-13T06:18:44Z |
| format | Article |
| id | doaj.art-7f4108049c1546de856fc183e56dd421 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-13T06:18:44Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-7f4108049c1546de856fc183e56dd4212023-06-10T04:28:11ZengElsevierHeliyon2405-84402023-06-0196e16866Fenugreek seed ethanolic extract inhibited formation of advanced glycation end products via scavenging reactive carbonyl intermediatesLaila Anwar0Syed Abid Ali1Sana Khan2Mir Muhammad Uzairullah3Nazish Mustafa4Urooj Anwer Ali5Faheema Siddiqui6Huma Aslam Bhatti7Syed Jawad Rehmani8Ghulam Abbas9H.E.J. Research Institute of Chemistry, ICCBS, University of Karachi, Karachi, Pakistan; Faculty of Pharmacy, Hamdard University, Karachi, PakistanH.E.J. Research Institute of Chemistry, ICCBS, University of Karachi, Karachi, PakistanH.E.J. Research Institute of Chemistry, ICCBS, University of Karachi, Karachi, PakistanH.E.J. Research Institute of Chemistry, ICCBS, University of Karachi, Karachi, PakistanDr. Panjwani Center for Molecular Medicine & Drug Research, ICCBS, University of Karachi, Karachi, PakistanCollege of Physicians and Surgeons, Karachi, PakistanFaculty of Pharmacy, DUHS, Karachi, PakistanH.E.J. Research Institute of Chemistry, ICCBS, University of Karachi, Karachi, PakistanCollege of Physicians and Surgeons, Karachi, PakistanDepartment of Pharmacology, Faculty of Pharmacy, Ziauddin University, Karachi, Pakistan; Corresponding author.Senescence is a natural phenomenon of growing old. It accelerates under certain conditions like diabetes mellitus resulting in early decline of bodily functions, which can be avoided by many claimed functional foods. The present study aims to investigate the anti-aging ability of Fenugreek seeds (Trigonellafoenum-graecum); a common ingredient of Indo-Pak cuisines. Briefly, the Fenugreek seeds extract (FgSE) in concentrationsof0.1, 0.5 and 1 mg/ml inhibited the formation of Advanced Glycation End products (AGEs) and fructosamine adducts in Bovine serum albumin (BSA)/fructose model in vitro. The BSA conformational analysis via Circular Dichorism and Congo red assays showed that it preserves secondary structure of BSA in aforementioned model. Although mechanistic studies revealed insignificant lysine blocking ability of Fenugreek by OPA assay, however carbonyl entrapping was found to be 24%, 34% and 42% at 0.1, 0.5 and 1 mg/ml, respectively. In vivo model of High Fructose diet (HFD) induced glycation, FgSE treatment in doses of 10, 25 & 50 mg/kg markedly improved Escape latency (p < 0.01) and preserved cognition in Morris Water Maze. Our data further exhibits significant decrease of CML (Nε-carboxymethyl lysine) levels in serum and hippocampus byFgSE treatment in comparison with HFD group. Therefore, we deduced that FgSE prevents glycation-induced memory decline via entrapping the reactive carbonyl intermediates, formed during production of AGEs. Hence, as a promising functional food it slows down the harmful process of glycation and aging associated morbidities.http://www.sciencedirect.com/science/article/pii/S2405844023040732Carbonyl trappingGlycationAnti-aging functional foodsTrigonellafoenum-graecum |
| spellingShingle | Laila Anwar Syed Abid Ali Sana Khan Mir Muhammad Uzairullah Nazish Mustafa Urooj Anwer Ali Faheema Siddiqui Huma Aslam Bhatti Syed Jawad Rehmani Ghulam Abbas Fenugreek seed ethanolic extract inhibited formation of advanced glycation end products via scavenging reactive carbonyl intermediates Heliyon Carbonyl trapping Glycation Anti-aging functional foods Trigonellafoenum-graecum |
| title | Fenugreek seed ethanolic extract inhibited formation of advanced glycation end products via scavenging reactive carbonyl intermediates |
| title_full | Fenugreek seed ethanolic extract inhibited formation of advanced glycation end products via scavenging reactive carbonyl intermediates |
| title_fullStr | Fenugreek seed ethanolic extract inhibited formation of advanced glycation end products via scavenging reactive carbonyl intermediates |
| title_full_unstemmed | Fenugreek seed ethanolic extract inhibited formation of advanced glycation end products via scavenging reactive carbonyl intermediates |
| title_short | Fenugreek seed ethanolic extract inhibited formation of advanced glycation end products via scavenging reactive carbonyl intermediates |
| title_sort | fenugreek seed ethanolic extract inhibited formation of advanced glycation end products via scavenging reactive carbonyl intermediates |
| topic | Carbonyl trapping Glycation Anti-aging functional foods Trigonellafoenum-graecum |
| url | http://www.sciencedirect.com/science/article/pii/S2405844023040732 |
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