A CRISPR-based chromosomal-separation technique for Escherichia coli

Abstract Background Natural life systems can be significantly modified at the genomic scale by human intervention, demonstrating the great innovation capacity of genome engineering. Large epi-chromosomal DNA structures were established in Escherichia coli cells, but some of these methods were inconv...

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Bibliographic Details
Main Authors: Junchang Su, Pengju Wang, Ju Li, Dongdong Zhao, Siwei Li, Feiyu Fan, Zhubo Dai, Xiaoping Liao, Zhitao Mao, Chunzhi Zhang, Changhao Bi, Xueli Zhang
Format: Article
Language:English
Published: BMC 2022-11-01
Series:Microbial Cell Factories
Subjects:
Online Access:https://doi.org/10.1186/s12934-022-01957-4