Effects of Different Divalent Cation Hydrothermal Treatments of Titanium Implant Surfaces for Epithelial Tissue Sealing
The improvement of peri-implant epithelium (PIE) adhesion to titanium (Ti) may promote Ti dental implant stability. This study aims to investigate whether there is a positive effect of Ti hydrothermally treated (HT) with calcium chloride (CaCl<sub>2</sub>), zinc chloride (ZnCl<sub>...
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| author | Xudiyang Zhou Ikiru Atsuta Yasunori Ayukawa Ikue Narimatsu Tianren Zhou Jiangqi Hu Kiyoshi Koyano |
| author_facet | Xudiyang Zhou Ikiru Atsuta Yasunori Ayukawa Ikue Narimatsu Tianren Zhou Jiangqi Hu Kiyoshi Koyano |
| author_sort | Xudiyang Zhou |
| collection | DOAJ |
| description | The improvement of peri-implant epithelium (PIE) adhesion to titanium (Ti) may promote Ti dental implant stability. This study aims to investigate whether there is a positive effect of Ti hydrothermally treated (HT) with calcium chloride (CaCl<sub>2</sub>), zinc chloride (ZnCl<sub>2</sub>), and strontium chloride (SrCl<sub>2</sub>) on promoting PIE sealing. We analyzed the response of a rat oral epithelial cell (OEC) culture and performed an in vivo study in which the maxillary right first molars of rats were extracted and replaced with calcium (Ca)-HT, zinc (Zn)-HT, strontium (Sr)-HT, or non-treated control (Cont) implants. The OEC adhesion on Ca-HT and Zn-HT Ti plates had a higher expression of adhesion proteins than cells on the Cont and Sr-HT Ti plates. Additionally, the implant PIE of the Ca-HT and Zn-HT groups revealed better expression of immunoreactive laminin-332 (Ln-322) at 2 weeks after implantation. The Ca-HT and Zn-HT groups also showed better attachment at the implant–PIE interface, which inhibited horseradish peroxidase penetration. These results demonstrated that the divalent cations of Ca (Ca<sup>2+</sup>) and Zn (Zn<sup>2+</sup>)-HT improve the integration of epithelium around the implant, which may facilitate the creation of a soft barrier around the implant to protect it from foreign body penetration. |
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| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-03-10T20:11:32Z |
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| spelling | doaj.art-7f7920826d574312baa3a584d1c952592023-11-19T22:53:05ZengMDPI AGMaterials1996-19442020-04-01139203810.3390/ma13092038Effects of Different Divalent Cation Hydrothermal Treatments of Titanium Implant Surfaces for Epithelial Tissue SealingXudiyang Zhou0Ikiru Atsuta1Yasunori Ayukawa2Ikue Narimatsu3Tianren Zhou4Jiangqi Hu5Kiyoshi Koyano6Section of Implant and Rehabilitative Dentistry, Division of Oral Rehabilitation, Faculty of Dental Science, Kyushu University, Fukuoka 812-8582, JapanDivision of Advanced Dental Devices and Therapeutics, Faculty of Dental Science, Kyushu University, Fukuoka 812-8582, JapanSection of Implant and Rehabilitative Dentistry, Division of Oral Rehabilitation, Faculty of Dental Science, Kyushu University, Fukuoka 812-8582, JapanSection of Implant and Rehabilitative Dentistry, Division of Oral Rehabilitation, Faculty of Dental Science, Kyushu University, Fukuoka 812-8582, JapanSection of Implant and Rehabilitative Dentistry, Division of Oral Rehabilitation, Faculty of Dental Science, Kyushu University, Fukuoka 812-8582, JapanSection of Implant and Rehabilitative Dentistry, Division of Oral Rehabilitation, Faculty of Dental Science, Kyushu University, Fukuoka 812-8582, JapanSection of Implant and Rehabilitative Dentistry, Division of Oral Rehabilitation, Faculty of Dental Science, Kyushu University, Fukuoka 812-8582, JapanThe improvement of peri-implant epithelium (PIE) adhesion to titanium (Ti) may promote Ti dental implant stability. This study aims to investigate whether there is a positive effect of Ti hydrothermally treated (HT) with calcium chloride (CaCl<sub>2</sub>), zinc chloride (ZnCl<sub>2</sub>), and strontium chloride (SrCl<sub>2</sub>) on promoting PIE sealing. We analyzed the response of a rat oral epithelial cell (OEC) culture and performed an in vivo study in which the maxillary right first molars of rats were extracted and replaced with calcium (Ca)-HT, zinc (Zn)-HT, strontium (Sr)-HT, or non-treated control (Cont) implants. The OEC adhesion on Ca-HT and Zn-HT Ti plates had a higher expression of adhesion proteins than cells on the Cont and Sr-HT Ti plates. Additionally, the implant PIE of the Ca-HT and Zn-HT groups revealed better expression of immunoreactive laminin-332 (Ln-322) at 2 weeks after implantation. The Ca-HT and Zn-HT groups also showed better attachment at the implant–PIE interface, which inhibited horseradish peroxidase penetration. These results demonstrated that the divalent cations of Ca (Ca<sup>2+</sup>) and Zn (Zn<sup>2+</sup>)-HT improve the integration of epithelium around the implant, which may facilitate the creation of a soft barrier around the implant to protect it from foreign body penetration.https://www.mdpi.com/1996-1944/13/9/2038divalent cationshydrothermal treatmenttitanium dental implantepithelial cells and tissueLaminin-332 |
| spellingShingle | Xudiyang Zhou Ikiru Atsuta Yasunori Ayukawa Ikue Narimatsu Tianren Zhou Jiangqi Hu Kiyoshi Koyano Effects of Different Divalent Cation Hydrothermal Treatments of Titanium Implant Surfaces for Epithelial Tissue Sealing Materials divalent cations hydrothermal treatment titanium dental implant epithelial cells and tissue Laminin-332 |
| title | Effects of Different Divalent Cation Hydrothermal Treatments of Titanium Implant Surfaces for Epithelial Tissue Sealing |
| title_full | Effects of Different Divalent Cation Hydrothermal Treatments of Titanium Implant Surfaces for Epithelial Tissue Sealing |
| title_fullStr | Effects of Different Divalent Cation Hydrothermal Treatments of Titanium Implant Surfaces for Epithelial Tissue Sealing |
| title_full_unstemmed | Effects of Different Divalent Cation Hydrothermal Treatments of Titanium Implant Surfaces for Epithelial Tissue Sealing |
| title_short | Effects of Different Divalent Cation Hydrothermal Treatments of Titanium Implant Surfaces for Epithelial Tissue Sealing |
| title_sort | effects of different divalent cation hydrothermal treatments of titanium implant surfaces for epithelial tissue sealing |
| topic | divalent cations hydrothermal treatment titanium dental implant epithelial cells and tissue Laminin-332 |
| url | https://www.mdpi.com/1996-1944/13/9/2038 |
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