Transcriptomic analysis provides a new insight: Oleuropein reverses high glucose-induced osteogenic inhibition in bone marrow mesenchymal stem cells via Wnt10b activation
Adverse events of diabetes mellitus (DM) include bone damages, such as the increased incidence of osteoporosis and bone fractures, which are known as diabetic osteopathy. The pathogenic mechanism of diabetic osteopathy is complex, and hyperglycemia is a vital cause involved in it. Bone marrow mesenc...
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Frontiers Media S.A.
2022-08-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2022.990507/full |
| _version_ | 1798036573870096384 |
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| author | An Lao Yu Chen Yiting Sun Tiange Wang Kaili Lin Jiaqiang Liu Jianyong Wu |
| author_facet | An Lao Yu Chen Yiting Sun Tiange Wang Kaili Lin Jiaqiang Liu Jianyong Wu |
| author_sort | An Lao |
| collection | DOAJ |
| description | Adverse events of diabetes mellitus (DM) include bone damages, such as the increased incidence of osteoporosis and bone fractures, which are known as diabetic osteopathy. The pathogenic mechanism of diabetic osteopathy is complex, and hyperglycemia is a vital cause involved in it. Bone marrow mesenchymal stem cells (BMSCs) exert a significant effect on bone formation. Therefore, in this paper, transcriptomic changes of BMSCs cultured in high glucose (35 mM) for 30 days are mainly investigated. In addition, 794 up-regulated genes and 1,162 down-regulated genes were identified. Then, biological functions of the differentially expressed genes in the high glucose microenvironment were investigated by two kinds of functional analyses. Gene Set Enrichment Analysis was also applied to focus on the significant gene sets and it is found that Wnt10b expression witnessed a remarkable decrease in BMSCs under the high glucose microenvironment. At last, in vitro experiments revealed that oleuropein effectively reversed high glucose-induced osteogenic inhibition via activating Wnt10b in BMSCs. |
| first_indexed | 2024-04-11T21:14:52Z |
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| id | doaj.art-7f8506dffe704b2d8d49a5910e0deb37 |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-04-11T21:14:52Z |
| publishDate | 2022-08-01 |
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| spelling | doaj.art-7f8506dffe704b2d8d49a5910e0deb372022-12-22T04:02:51ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852022-08-011010.3389/fbioe.2022.990507990507Transcriptomic analysis provides a new insight: Oleuropein reverses high glucose-induced osteogenic inhibition in bone marrow mesenchymal stem cells via Wnt10b activationAn Lao0Yu Chen1Yiting Sun2Tiange Wang3Kaili Lin4Jiaqiang Liu5Jianyong Wu6Department of Stomatology, Xin Hua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaShanghai Key Laboratory of Stomatology, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Department of Oral and Cranio-maxillofacial Surgery, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University, Shanghai, ChinaShanghai Key Laboratory of Stomatology, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Department of Oral and Cranio-maxillofacial Surgery, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University, Shanghai, ChinaShanghai Key Laboratory of Stomatology, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Department of Oral and Cranio-maxillofacial Surgery, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University, Shanghai, ChinaShanghai Key Laboratory of Stomatology, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Department of Oral and Cranio-maxillofacial Surgery, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University, Shanghai, ChinaShanghai Key Laboratory of Stomatology, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Department of Oral and Cranio-maxillofacial Surgery, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Stomatology, Xin Hua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaAdverse events of diabetes mellitus (DM) include bone damages, such as the increased incidence of osteoporosis and bone fractures, which are known as diabetic osteopathy. The pathogenic mechanism of diabetic osteopathy is complex, and hyperglycemia is a vital cause involved in it. Bone marrow mesenchymal stem cells (BMSCs) exert a significant effect on bone formation. Therefore, in this paper, transcriptomic changes of BMSCs cultured in high glucose (35 mM) for 30 days are mainly investigated. In addition, 794 up-regulated genes and 1,162 down-regulated genes were identified. Then, biological functions of the differentially expressed genes in the high glucose microenvironment were investigated by two kinds of functional analyses. Gene Set Enrichment Analysis was also applied to focus on the significant gene sets and it is found that Wnt10b expression witnessed a remarkable decrease in BMSCs under the high glucose microenvironment. At last, in vitro experiments revealed that oleuropein effectively reversed high glucose-induced osteogenic inhibition via activating Wnt10b in BMSCs.https://www.frontiersin.org/articles/10.3389/fbioe.2022.990507/fulltranscriptome sequencingBMSCshigh glucose microenvironmentWnt10boleuropein |
| spellingShingle | An Lao Yu Chen Yiting Sun Tiange Wang Kaili Lin Jiaqiang Liu Jianyong Wu Transcriptomic analysis provides a new insight: Oleuropein reverses high glucose-induced osteogenic inhibition in bone marrow mesenchymal stem cells via Wnt10b activation Frontiers in Bioengineering and Biotechnology transcriptome sequencing BMSCs high glucose microenvironment Wnt10b oleuropein |
| title | Transcriptomic analysis provides a new insight: Oleuropein reverses high glucose-induced osteogenic inhibition in bone marrow mesenchymal stem cells via Wnt10b activation |
| title_full | Transcriptomic analysis provides a new insight: Oleuropein reverses high glucose-induced osteogenic inhibition in bone marrow mesenchymal stem cells via Wnt10b activation |
| title_fullStr | Transcriptomic analysis provides a new insight: Oleuropein reverses high glucose-induced osteogenic inhibition in bone marrow mesenchymal stem cells via Wnt10b activation |
| title_full_unstemmed | Transcriptomic analysis provides a new insight: Oleuropein reverses high glucose-induced osteogenic inhibition in bone marrow mesenchymal stem cells via Wnt10b activation |
| title_short | Transcriptomic analysis provides a new insight: Oleuropein reverses high glucose-induced osteogenic inhibition in bone marrow mesenchymal stem cells via Wnt10b activation |
| title_sort | transcriptomic analysis provides a new insight oleuropein reverses high glucose induced osteogenic inhibition in bone marrow mesenchymal stem cells via wnt10b activation |
| topic | transcriptome sequencing BMSCs high glucose microenvironment Wnt10b oleuropein |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2022.990507/full |
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