Pitfalls associated with lipophilic fluorophore staining of extracellular vesicles for uptake studies
Post-staining of extracellular vesicles (EVs) with lipid-anchored fluorophores (LAFs) such as PKH67 is a widely used strategy for studying EVs but it is associated with several pitfalls. The pitfalls discussed in this commentary are related to LAF labelling of non-EV species due to (1) lipoprotein c...
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| Format: | Article |
| Language: | English |
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Wiley
2019-12-01
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| Series: | Journal of Extracellular Vesicles |
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| Online Access: | http://dx.doi.org/10.1080/20013078.2019.1582237 |