Antimalarial drugs disrupt ion homeostasis in malarial parasites
Plasmodium chabaudi malaria parasite organelles are major elements for ion homeostasis and cellular signaling and also target for antimalarial drugs. By using confocal imaging of intraerythrocytic parasites we demonstrated that the dye acridine orange (AO) is accumulated into P. chabaudi subcellular...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2007-06-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762007000300012 |
| _version_ | 1797718178653011968 |
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| author | Marcos L Gazarini Carlos AO Sigolo Regina P Markus Andrew P Thomas Célia RS Garcia |
| author_facet | Marcos L Gazarini Carlos AO Sigolo Regina P Markus Andrew P Thomas Célia RS Garcia |
| author_sort | Marcos L Gazarini |
| collection | DOAJ |
| description | Plasmodium chabaudi malaria parasite organelles are major elements for ion homeostasis and cellular signaling and also target for antimalarial drugs. By using confocal imaging of intraerythrocytic parasites we demonstrated that the dye acridine orange (AO) is accumulated into P. chabaudi subcellular compartments. The AO could be released from the parasite organelles by collapsing the pH gradient with the K+/H+ ionophore nigericin (20 µM), or by inhibiting the H+-pump with bafilomycin (4 µM). Similarly, in isolated parasites loaded with calcium indicator Fluo 3-AM, bafilomycin caused calcium mobilization of the acidic calcium pool that could also be release with nigericin. Interestingly after complete release of the acidic compartments, addition of thapsigargin at 10 µM was still effective in releasing parasite intracellular calcium stores in parasites at trophozoite stage. The addition of antimalarial drugs chloroquine and artemisinin resulted in AO release from acidic compartments and also affected maintenance of calcium in ER store by using different drug concentrations. |
| first_indexed | 2024-03-12T08:47:52Z |
| format | Article |
| id | doaj.art-7fad1867a27b44fe8f7bd71a01fca28f |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T08:47:52Z |
| publishDate | 2007-06-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-7fad1867a27b44fe8f7bd71a01fca28f2023-09-02T16:25:25ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80602007-06-011023329334Antimalarial drugs disrupt ion homeostasis in malarial parasitesMarcos L GazariniCarlos AO SigoloRegina P MarkusAndrew P ThomasCélia RS GarciaPlasmodium chabaudi malaria parasite organelles are major elements for ion homeostasis and cellular signaling and also target for antimalarial drugs. By using confocal imaging of intraerythrocytic parasites we demonstrated that the dye acridine orange (AO) is accumulated into P. chabaudi subcellular compartments. The AO could be released from the parasite organelles by collapsing the pH gradient with the K+/H+ ionophore nigericin (20 µM), or by inhibiting the H+-pump with bafilomycin (4 µM). Similarly, in isolated parasites loaded with calcium indicator Fluo 3-AM, bafilomycin caused calcium mobilization of the acidic calcium pool that could also be release with nigericin. Interestingly after complete release of the acidic compartments, addition of thapsigargin at 10 µM was still effective in releasing parasite intracellular calcium stores in parasites at trophozoite stage. The addition of antimalarial drugs chloroquine and artemisinin resulted in AO release from acidic compartments and also affected maintenance of calcium in ER store by using different drug concentrations.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762007000300012malariaPlasmodium chabaudicalciumchloroquineartemisininacidic compartment |
| spellingShingle | Marcos L Gazarini Carlos AO Sigolo Regina P Markus Andrew P Thomas Célia RS Garcia Antimalarial drugs disrupt ion homeostasis in malarial parasites Memorias do Instituto Oswaldo Cruz malaria Plasmodium chabaudi calcium chloroquine artemisinin acidic compartment |
| title | Antimalarial drugs disrupt ion homeostasis in malarial parasites |
| title_full | Antimalarial drugs disrupt ion homeostasis in malarial parasites |
| title_fullStr | Antimalarial drugs disrupt ion homeostasis in malarial parasites |
| title_full_unstemmed | Antimalarial drugs disrupt ion homeostasis in malarial parasites |
| title_short | Antimalarial drugs disrupt ion homeostasis in malarial parasites |
| title_sort | antimalarial drugs disrupt ion homeostasis in malarial parasites |
| topic | malaria Plasmodium chabaudi calcium chloroquine artemisinin acidic compartment |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762007000300012 |
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