Efficient support of virus-like particle assembly by the HIV-1 packaging signal
The principal structural component of a retrovirus particle is the Gag protein. Retroviral genomic RNAs contain a ‘packaging signal’ (‘Ψ') and are packaged in virus particles with very high selectivity. However, if no genomic RNA is present, Gag assembles into particles containing cellular mRNA...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
eLife Sciences Publications Ltd
2018-08-01
|
| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/38438 |
| _version_ | 1818028319236423680 |
|---|---|
| author | Mauricio Comas-Garcia Tomas Kroupa Siddhartha AK Datta Demetria P Harvin Wei-Shau Hu Alan Rein |
| author_facet | Mauricio Comas-Garcia Tomas Kroupa Siddhartha AK Datta Demetria P Harvin Wei-Shau Hu Alan Rein |
| author_sort | Mauricio Comas-Garcia |
| collection | DOAJ |
| description | The principal structural component of a retrovirus particle is the Gag protein. Retroviral genomic RNAs contain a ‘packaging signal’ (‘Ψ') and are packaged in virus particles with very high selectivity. However, if no genomic RNA is present, Gag assembles into particles containing cellular mRNA molecules. The mechanism by which genomic RNA is normally selected during virus assembly is not understood. We previously reported (Comas-Garcia et al., 2017) that at physiological ionic strength, recombinant HIV-1 Gag binds with similar affinities to RNAs with or without Ψ, and proposed that genomic RNA is selectively packaged because binding to Ψ initiates particle assembly more efficiently than other RNAs. We now present data directly supporting this hypothesis. We also show that one or more short stretches of unpaired G residues are important elements of Ψ; Ψ may not be localized to a single structural element, but is probably distributed over >100 bases. |
| first_indexed | 2024-12-10T05:01:54Z |
| format | Article |
| id | doaj.art-7fbaa5c7b160404090290a40ca2bb2f6 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-12-10T05:01:54Z |
| publishDate | 2018-08-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-7fbaa5c7b160404090290a40ca2bb2f62022-12-22T02:01:23ZengeLife Sciences Publications LtdeLife2050-084X2018-08-01710.7554/eLife.38438Efficient support of virus-like particle assembly by the HIV-1 packaging signalMauricio Comas-Garcia0https://orcid.org/0000-0002-7733-5138Tomas Kroupa1https://orcid.org/0000-0002-5996-9057Siddhartha AK Datta2Demetria P Harvin3Wei-Shau Hu4Alan Rein5https://orcid.org/0000-0002-8273-546XHIV Dynamics and Replication Program, Center for Cancer Research, National Cancer Institute, Frederick, United StatesHIV Dynamics and Replication Program, Center for Cancer Research, National Cancer Institute, Frederick, United StatesHIV Dynamics and Replication Program, Center for Cancer Research, National Cancer Institute, Frederick, United StatesHIV Dynamics and Replication Program, Center for Cancer Research, National Cancer Institute, Frederick, United StatesHIV Dynamics and Replication Program, Center for Cancer Research, National Cancer Institute, Frederick, United StatesHIV Dynamics and Replication Program, Center for Cancer Research, National Cancer Institute, Frederick, United StatesThe principal structural component of a retrovirus particle is the Gag protein. Retroviral genomic RNAs contain a ‘packaging signal’ (‘Ψ') and are packaged in virus particles with very high selectivity. However, if no genomic RNA is present, Gag assembles into particles containing cellular mRNA molecules. The mechanism by which genomic RNA is normally selected during virus assembly is not understood. We previously reported (Comas-Garcia et al., 2017) that at physiological ionic strength, recombinant HIV-1 Gag binds with similar affinities to RNAs with or without Ψ, and proposed that genomic RNA is selectively packaged because binding to Ψ initiates particle assembly more efficiently than other RNAs. We now present data directly supporting this hypothesis. We also show that one or more short stretches of unpaired G residues are important elements of Ψ; Ψ may not be localized to a single structural element, but is probably distributed over >100 bases.https://elifesciences.org/articles/38438HIVvirus assemblyRNA |
| spellingShingle | Mauricio Comas-Garcia Tomas Kroupa Siddhartha AK Datta Demetria P Harvin Wei-Shau Hu Alan Rein Efficient support of virus-like particle assembly by the HIV-1 packaging signal eLife HIV virus assembly RNA |
| title | Efficient support of virus-like particle assembly by the HIV-1 packaging signal |
| title_full | Efficient support of virus-like particle assembly by the HIV-1 packaging signal |
| title_fullStr | Efficient support of virus-like particle assembly by the HIV-1 packaging signal |
| title_full_unstemmed | Efficient support of virus-like particle assembly by the HIV-1 packaging signal |
| title_short | Efficient support of virus-like particle assembly by the HIV-1 packaging signal |
| title_sort | efficient support of virus like particle assembly by the hiv 1 packaging signal |
| topic | HIV virus assembly RNA |
| url | https://elifesciences.org/articles/38438 |
| work_keys_str_mv | AT mauriciocomasgarcia efficientsupportofviruslikeparticleassemblybythehiv1packagingsignal AT tomaskroupa efficientsupportofviruslikeparticleassemblybythehiv1packagingsignal AT siddharthaakdatta efficientsupportofviruslikeparticleassemblybythehiv1packagingsignal AT demetriapharvin efficientsupportofviruslikeparticleassemblybythehiv1packagingsignal AT weishauhu efficientsupportofviruslikeparticleassemblybythehiv1packagingsignal AT alanrein efficientsupportofviruslikeparticleassemblybythehiv1packagingsignal |