Two New Plasmid Post-segregational Killing Mechanisms for the Implementation of Synthetic Gene Networks in Escherichia coli

Summary: Plasmids are the workhorse of both industrial biotechnology and synthetic biology, but ensuring they remain in bacterial cells is a challenge. Antibiotic selection cannot be used to stabilize plasmids in most real-world applications, and inserting dynamical gene networks into the genome rem...

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Detalhes bibliográficos
Main Authors: Alex J.H. Fedorec, Tanel Ozdemir, Anjali Doshi, Yan-Kay Ho, Luca Rosa, Jack Rutter, Oscar Velazquez, Vitor B. Pinheiro, Tal Danino, Chris P. Barnes
Formato: Artigo
Idioma:English
Publicado em: Elsevier 2019-04-01
Colecção:iScience
Acesso em linha:http://www.sciencedirect.com/science/article/pii/S2589004219300859