Transcriptomic and phenotype analysis revealed the role of rpoS in stress resistance and virulence of a novel ST3355 ESBL-producing hypervirulent Klebsiella pneumoniae isolate
IntroductionAn extended-spectrum beta-lactamase (ESBL)-hypervirulent Klebsiella pneumoniae (HvKP) strain HKE9 was isolated from the blood in an outpatient.MethodsThe effect of the global regulatory factor RpoS on antimicrobial resistance, pathogenicity, and environmental adaptability was elucidated....
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Frontiers Media S.A.
2023-10-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2023.1259472/full |
| _version_ | 1827786358817554432 |
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| author | Yi Zhou Yue Cheng Tianyou Ma Jun Wang Jun Wang Shaoru Li Jingdan Wang Lei Han Xinyao Hou Xinxin Ma Sijin Jiang Pu Li Jia Lv Bei Han Rong Da |
| author_facet | Yi Zhou Yue Cheng Tianyou Ma Jun Wang Jun Wang Shaoru Li Jingdan Wang Lei Han Xinyao Hou Xinxin Ma Sijin Jiang Pu Li Jia Lv Bei Han Rong Da |
| author_sort | Yi Zhou |
| collection | DOAJ |
| description | IntroductionAn extended-spectrum beta-lactamase (ESBL)-hypervirulent Klebsiella pneumoniae (HvKP) strain HKE9 was isolated from the blood in an outpatient.MethodsThe effect of the global regulatory factor RpoS on antimicrobial resistance, pathogenicity, and environmental adaptability was elucidated.ResultsHKE9 is a novel ST3355 (K20/O2a) hypervirulent strain with a positive string test and resistant to cephems except cefotetan. It has a genome size of 5.6M, including two plasmids. CTX-M-15 was found in plasmid 2, and only ompk37 was found in the chromosome. HKE9 could produce bacterial siderophores, and genes of enterobactin, yersiniabactin, aerobactin, and salmochelin have been retrieved in the genome. As a global regulatory factor, knockout of rpoS did not change antimicrobial resistance or hemolytic phenotype while increasing the virulence to Galleria mellonella larvae and showing higher viscosity. Moreover, rpoS knockout can increase bacterial competitiveness and cell adhesion ability. Interestingly, HKE9-M-rpoS decreased resistance to acidic pH, high osmotic pressure, heat shock, and ultraviolet and became sensitive to disinfectants (H2O2, alcohol, and sodium hypochlorite). Although there were 13 Type 6 secretion system (T6SS) core genes divided into two segments with tle1 between segments in the chromosome, transcriptomic analysis showed that rpoS negatively regulated T4SS located on plasmid 2, type 1, and type 3 fimbriae and positively regulate genes responsible for acidic response, hyperosmotic pressure, heat shock, oxidative stress, alcohol and hypochlorous acid metabolism, and quorum sensing.DiscussionHere, this novel ST3355 ESBL-HvKP strain HKE9 may spread via various clonal types. The important regulation effect of rpoS is the enhanced tolerance and resistance to environmental stress and disinfectants, which may be at the cost of reducing virulence and regulated by T4SS. |
| first_indexed | 2024-03-11T16:33:19Z |
| format | Article |
| id | doaj.art-7fca79b43707425695a984fbf400bd8a |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-03-11T16:33:19Z |
| publishDate | 2023-10-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-7fca79b43707425695a984fbf400bd8a2023-10-23T21:04:19ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882023-10-011310.3389/fcimb.2023.12594721259472Transcriptomic and phenotype analysis revealed the role of rpoS in stress resistance and virulence of a novel ST3355 ESBL-producing hypervirulent Klebsiella pneumoniae isolateYi Zhou0Yue Cheng1Tianyou Ma2Jun Wang3Jun Wang4Shaoru Li5Jingdan Wang6Lei Han7Xinyao Hou8Xinxin Ma9Sijin Jiang10Pu Li11Jia Lv12Bei Han13Rong Da14School of Public Health, Health Science Center, Xi’an Jiaotong University, Xi’an, ChinaSchool of Public Health, Health Science Center, Xi’an Jiaotong University, Xi’an, ChinaSchool of Public Health, Health Science Center, Xi’an Jiaotong University, Xi’an, ChinaSchool of Public Health, Health Science Center, Xi’an Jiaotong University, Xi’an, ChinaDepartment of Microbiology Laboratory, Tongchuan Center for Disease Control and Prevention, Tongchuan, Shaanxi, ChinaSchool of Public Health, Health Science Center, Xi’an Jiaotong University, Xi’an, ChinaSchool of Public Health, Health Science Center, Xi’an Jiaotong University, Xi’an, ChinaSchool of Basic Medicine, Health Science Center, Xi’an Jiaotong University, Xi’an, ChinaSchool of Public Health, Health Science Center, Xi’an Jiaotong University, Xi’an, ChinaSchool of Public Health, Health Science Center, Xi’an Jiaotong University, Xi’an, ChinaSchool of Public Health, Health Science Center, Xi’an Jiaotong University, Xi’an, ChinaSchool of Public Health, Health Science Center, Xi’an Jiaotong University, Xi’an, ChinaSchool of Public Health, Health Science Center, Xi’an Jiaotong University, Xi’an, ChinaSchool of Public Health, Health Science Center, Xi’an Jiaotong University, Xi’an, ChinaDepartment of Clinical Laboratory, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, ChinaIntroductionAn extended-spectrum beta-lactamase (ESBL)-hypervirulent Klebsiella pneumoniae (HvKP) strain HKE9 was isolated from the blood in an outpatient.MethodsThe effect of the global regulatory factor RpoS on antimicrobial resistance, pathogenicity, and environmental adaptability was elucidated.ResultsHKE9 is a novel ST3355 (K20/O2a) hypervirulent strain with a positive string test and resistant to cephems except cefotetan. It has a genome size of 5.6M, including two plasmids. CTX-M-15 was found in plasmid 2, and only ompk37 was found in the chromosome. HKE9 could produce bacterial siderophores, and genes of enterobactin, yersiniabactin, aerobactin, and salmochelin have been retrieved in the genome. As a global regulatory factor, knockout of rpoS did not change antimicrobial resistance or hemolytic phenotype while increasing the virulence to Galleria mellonella larvae and showing higher viscosity. Moreover, rpoS knockout can increase bacterial competitiveness and cell adhesion ability. Interestingly, HKE9-M-rpoS decreased resistance to acidic pH, high osmotic pressure, heat shock, and ultraviolet and became sensitive to disinfectants (H2O2, alcohol, and sodium hypochlorite). Although there were 13 Type 6 secretion system (T6SS) core genes divided into two segments with tle1 between segments in the chromosome, transcriptomic analysis showed that rpoS negatively regulated T4SS located on plasmid 2, type 1, and type 3 fimbriae and positively regulate genes responsible for acidic response, hyperosmotic pressure, heat shock, oxidative stress, alcohol and hypochlorous acid metabolism, and quorum sensing.DiscussionHere, this novel ST3355 ESBL-HvKP strain HKE9 may spread via various clonal types. The important regulation effect of rpoS is the enhanced tolerance and resistance to environmental stress and disinfectants, which may be at the cost of reducing virulence and regulated by T4SS.https://www.frontiersin.org/articles/10.3389/fcimb.2023.1259472/fullhypervirulent Klebsiella pneumoniaeESBLspathogenomicrpoSvirulenceenvironmental stress and disinfectants tolerance |
| spellingShingle | Yi Zhou Yue Cheng Tianyou Ma Jun Wang Jun Wang Shaoru Li Jingdan Wang Lei Han Xinyao Hou Xinxin Ma Sijin Jiang Pu Li Jia Lv Bei Han Rong Da Transcriptomic and phenotype analysis revealed the role of rpoS in stress resistance and virulence of a novel ST3355 ESBL-producing hypervirulent Klebsiella pneumoniae isolate Frontiers in Cellular and Infection Microbiology hypervirulent Klebsiella pneumoniae ESBLs pathogenomic rpoS virulence environmental stress and disinfectants tolerance |
| title | Transcriptomic and phenotype analysis revealed the role of rpoS in stress resistance and virulence of a novel ST3355 ESBL-producing hypervirulent Klebsiella pneumoniae isolate |
| title_full | Transcriptomic and phenotype analysis revealed the role of rpoS in stress resistance and virulence of a novel ST3355 ESBL-producing hypervirulent Klebsiella pneumoniae isolate |
| title_fullStr | Transcriptomic and phenotype analysis revealed the role of rpoS in stress resistance and virulence of a novel ST3355 ESBL-producing hypervirulent Klebsiella pneumoniae isolate |
| title_full_unstemmed | Transcriptomic and phenotype analysis revealed the role of rpoS in stress resistance and virulence of a novel ST3355 ESBL-producing hypervirulent Klebsiella pneumoniae isolate |
| title_short | Transcriptomic and phenotype analysis revealed the role of rpoS in stress resistance and virulence of a novel ST3355 ESBL-producing hypervirulent Klebsiella pneumoniae isolate |
| title_sort | transcriptomic and phenotype analysis revealed the role of rpos in stress resistance and virulence of a novel st3355 esbl producing hypervirulent klebsiella pneumoniae isolate |
| topic | hypervirulent Klebsiella pneumoniae ESBLs pathogenomic rpoS virulence environmental stress and disinfectants tolerance |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2023.1259472/full |
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