Effects of variation in sample storage conditions and swab order on 16S vaginal microbiome analyses

Effects of variation in sample storage conditions and swab order on 16S vaginal microbiome analyses

ABSTRACT Technical bias is a pressing issue in microbiome research, and variability can be introduced at any stage from sample collection to figure generation. In this study, we aim to reduce biases in studying the human vaginal microbiome by examining the impact of sample storage buffer and multipl...

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Bibliographic Details
Main Authors: Tanya Kumar, MacKenzie Bryant, Kalen Cantrell, Se Jin Song, Daniel McDonald, Helena M. Tubb, Sawyer Farmer, Amanda Lewis, Emily S. Lukacz, Linda Brubaker, Rob Knight
Format: Article
Language:English
Published: American Society for Microbiology 2024-01-01
Series:Microbiology Spectrum
Subjects:
vaginal microbiome
microbiome
sample storage
sample collection
preservation method
16S
Online Access:https://journals.asm.org/doi/10.1128/spectrum.03712-23

Internet

https://journals.asm.org/doi/10.1128/spectrum.03712-23

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