Mucosal and systemic immunization against tuberculosis by ISCOMATRIX nano adjuvant co-administered with alginate coated chitosan nanoparticles
Objective(s): BCG vaccine has no longer been appreciated to immunize against tuberculosis, worldwide, so novel appropriate adjuvants have been dedicated to improve immune responses. This study aimed to evaluate the immunomodulatory effects of ISCOMATRIX as an adjuvant to stimulate potent humoral and...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2023-10-01
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| Series: | Iranian Journal of Basic Medical Sciences |
| Subjects: | |
| Online Access: | https://ijbms.mums.ac.ir/article_22667_5e9cbf45793c425696386ac81eddba20.pdf |
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| author | Adel Najafi Kiarash Ghazvini Mojtaba Sankian Leila Gholami Sirvan Zare Arshid Youdefi-Avarvand Mohsen Tafaghodi |
| author_facet | Adel Najafi Kiarash Ghazvini Mojtaba Sankian Leila Gholami Sirvan Zare Arshid Youdefi-Avarvand Mohsen Tafaghodi |
| author_sort | Adel Najafi |
| collection | DOAJ |
| description | Objective(s): BCG vaccine has no longer been appreciated to immunize against tuberculosis, worldwide, so novel appropriate adjuvants have been dedicated to improve immune responses. This study aimed to evaluate the immunomodulatory effects of ISCOMATRIX as an adjuvant to stimulate potent humoral and cellular immune responses of the PPE17 loaded alginate coated nanoparticles through subcutaneous and intranasal vaccination. Materials and Methods: Size, polydispersity index, and morphology of the resulting colloidal particles were explored by dynamic light scattering (DLS). The cellular and/or humoral immune stimulation properties of ISCOMATRIX adjuvant were measured by measuring the level of IFNγ, IL-4, IL-17, and TGFβ in spleen cell cultures and IgG1 and IgG2a in serum and sIgA in nasal lavage of immunized mice, respectively. Results: The spherical cage-like particles of ISCOMATRIX adjuvant have optimal size of 59±6 nm appropriate for an immune adjuvant vaccine. ISCOMATRIX induced robust Th1 (IFN-γ) and IL-17 cytokine response also significant IgG2a and IgG1antibodies in both subcutaneous and intranasal routes and elicited mucosal sIgA response when administered intranasally. As a booster for BCG, ISCOMATRIX induced immune responses only in subcutaneous route. Conclusion: These findings indicate that ISCOMATRIX is a promising adjuvant with the potential for increasing cellular and humoral immunity both after subcutaneous and intranasal administration. |
| first_indexed | 2024-03-12T00:03:14Z |
| format | Article |
| id | doaj.art-7fda0c033f5046ea97532fd29407c508 |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-03-12T00:03:14Z |
| publishDate | 2023-10-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-7fda0c033f5046ea97532fd29407c5082023-09-17T06:59:56ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742023-10-0126101162116710.22038/ijbms.2023.67564.1480622667Mucosal and systemic immunization against tuberculosis by ISCOMATRIX nano adjuvant co-administered with alginate coated chitosan nanoparticlesAdel Najafi0Kiarash Ghazvini1Mojtaba Sankian2Leila Gholami3Sirvan Zare4Arshid Youdefi-Avarvand5Mohsen Tafaghodi6Clinical Microbiology Laboratory, Fatemieh hospital, Hamadan University of Medical Science, Hamedan, IranDepartment of Microbiology, Faculty of Medicine, Mashhad University of Medical Science, Mashhad, IranImmunology Research Center, Bu Ali Research Institute, Mashhad University of Medical Science, Mashhad, IranDepartment of Molecular Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, IranImmunology Research Center, Bu Ali Research Institute, Mashhad University of Medical Science, Mashhad, IranDepartment of Laboratory Sciences, School of Allied Medical Sciences, Ahavz Jundishapur University of Medical Sciences, Ahvaz, IranNanotechnology Research center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, IranObjective(s): BCG vaccine has no longer been appreciated to immunize against tuberculosis, worldwide, so novel appropriate adjuvants have been dedicated to improve immune responses. This study aimed to evaluate the immunomodulatory effects of ISCOMATRIX as an adjuvant to stimulate potent humoral and cellular immune responses of the PPE17 loaded alginate coated nanoparticles through subcutaneous and intranasal vaccination. Materials and Methods: Size, polydispersity index, and morphology of the resulting colloidal particles were explored by dynamic light scattering (DLS). The cellular and/or humoral immune stimulation properties of ISCOMATRIX adjuvant were measured by measuring the level of IFNγ, IL-4, IL-17, and TGFβ in spleen cell cultures and IgG1 and IgG2a in serum and sIgA in nasal lavage of immunized mice, respectively. Results: The spherical cage-like particles of ISCOMATRIX adjuvant have optimal size of 59±6 nm appropriate for an immune adjuvant vaccine. ISCOMATRIX induced robust Th1 (IFN-γ) and IL-17 cytokine response also significant IgG2a and IgG1antibodies in both subcutaneous and intranasal routes and elicited mucosal sIgA response when administered intranasally. As a booster for BCG, ISCOMATRIX induced immune responses only in subcutaneous route. Conclusion: These findings indicate that ISCOMATRIX is a promising adjuvant with the potential for increasing cellular and humoral immunity both after subcutaneous and intranasal administration.https://ijbms.mums.ac.ir/article_22667_5e9cbf45793c425696386ac81eddba20.pdfadjuvantchitosanimmune responseiscomatrixnanoparticle |
| spellingShingle | Adel Najafi Kiarash Ghazvini Mojtaba Sankian Leila Gholami Sirvan Zare Arshid Youdefi-Avarvand Mohsen Tafaghodi Mucosal and systemic immunization against tuberculosis by ISCOMATRIX nano adjuvant co-administered with alginate coated chitosan nanoparticles Iranian Journal of Basic Medical Sciences adjuvant chitosan immune response iscomatrix nanoparticle |
| title | Mucosal and systemic immunization against tuberculosis by ISCOMATRIX nano adjuvant co-administered with alginate coated chitosan nanoparticles |
| title_full | Mucosal and systemic immunization against tuberculosis by ISCOMATRIX nano adjuvant co-administered with alginate coated chitosan nanoparticles |
| title_fullStr | Mucosal and systemic immunization against tuberculosis by ISCOMATRIX nano adjuvant co-administered with alginate coated chitosan nanoparticles |
| title_full_unstemmed | Mucosal and systemic immunization against tuberculosis by ISCOMATRIX nano adjuvant co-administered with alginate coated chitosan nanoparticles |
| title_short | Mucosal and systemic immunization against tuberculosis by ISCOMATRIX nano adjuvant co-administered with alginate coated chitosan nanoparticles |
| title_sort | mucosal and systemic immunization against tuberculosis by iscomatrix nano adjuvant co administered with alginate coated chitosan nanoparticles |
| topic | adjuvant chitosan immune response iscomatrix nanoparticle |
| url | https://ijbms.mums.ac.ir/article_22667_5e9cbf45793c425696386ac81eddba20.pdf |
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