Biochemical Pulldown of mRNAs and Long Noncoding RNAs from Cellular Lysates Coupled with Mass Spectrometry to Identify Protein Binding Partners

RNA binding proteins (RBPs) interact with cellular mRNAs, controlling various steps throughout the lifetime of these transcripts, including transcription, cellular transport, subcellular localization, translation and degradation. In addition to binding mRNA transcripts, a growing number of RBPs are...

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Bibliographic Details
Main Authors: Anca Savulescu, Stoyan Stoychev, Sipho Mamputha, Musa Mhlanga
Format: Article
Language:English
Published: Bio-protocol LLC 2020-06-01
Series:Bio-Protocol
Online Access:https://bio-protocol.org/en/bpdetail?id=3639&type=0