Trophoblast retrieval from the cervical canal to predict abnormal pregnancy early in gestation: a pilot study
Abstract Background The current detection of fetal chromosomal abnormalities by non-invasive prenatal testing (NIPT) mainly relies on the cell free DNA(cfDNA) in the maternal blood. However, a gestational age of less than 12 weeks or a high maternal BMI affects cfDNA fetal fraction and further the d...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-03-01
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| Series: | BMC Pregnancy and Childbirth |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12884-023-05499-4 |
| _version_ | 1827982416784916480 |
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| author | Xiaoke Yang Liuyezi Du Yue Li Lin Liang Linlin Ma Shaowei Wang |
| author_facet | Xiaoke Yang Liuyezi Du Yue Li Lin Liang Linlin Ma Shaowei Wang |
| author_sort | Xiaoke Yang |
| collection | DOAJ |
| description | Abstract Background The current detection of fetal chromosomal abnormalities by non-invasive prenatal testing (NIPT) mainly relies on the cell free DNA(cfDNA) in the maternal blood. However, a gestational age of less than 12 weeks or a high maternal BMI affects cfDNA fetal fraction and further the detection by NIPT negatively. In this study, we aim to retrieve the trophoblast cells from the maternal cervix to develop a new sampling method for NIPT enabling an earlier use of NIPT. Methods We enrolled three patients who wanted to undergo induced abortion at Beijing Hospital between January 2022 and March 2022. Peripheral blood, cervix specimen, and the abortion tissue were collected and processed for each patient. Allele frequencies of the mutated gene loci of the maternal blood and the cervix sample were compared and the Sex Determining Region Y (SRY) gene was tested. Results The allele frequencies of the mutated gene loci showed no significant difference between the maternal blood and the cervix sample. But we successfully detected signal of the SRY gene in the cervix sample of the only patient carrying a male fetus. Conclusions The detection of the SRY gene in a cervix sample indicated a successful retrieval of trophoblast cells from the cervix canal. Further study needs to be conducted to verify our finding before its application to the clinical settings. |
| first_indexed | 2024-04-09T22:32:58Z |
| format | Article |
| id | doaj.art-800aced5ffe34abb8eada1c0a4331fab |
| institution | Directory Open Access Journal |
| issn | 1471-2393 |
| language | English |
| last_indexed | 2024-04-09T22:32:58Z |
| publishDate | 2023-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Pregnancy and Childbirth |
| spelling | doaj.art-800aced5ffe34abb8eada1c0a4331fab2023-03-22T12:39:08ZengBMCBMC Pregnancy and Childbirth1471-23932023-03-012311710.1186/s12884-023-05499-4Trophoblast retrieval from the cervical canal to predict abnormal pregnancy early in gestation: a pilot studyXiaoke Yang0Liuyezi Du1Yue Li2Lin Liang3Linlin Ma4Shaowei Wang5Department of Gynecology and Obstetrics, Beijing Hospital, National Center of Gerontology, Institute of Geriatric Medicine, Chinese Academy of Medical SciencesBeijing USCI Medical LaboratoryBeijing USCI Medical LaboratoryDepartment of Gynecology and Obstetrics, Beijing Hospital, National Center of Gerontology, Institute of Geriatric Medicine, Chinese Academy of Medical SciencesDepartment of Gynecology and Obstetrics, Beijing Hospital, National Center of Gerontology, Institute of Geriatric Medicine, Chinese Academy of Medical SciencesDepartment of Gynecology and Obstetrics, Beijing Hospital, National Center of Gerontology, Institute of Geriatric Medicine, Chinese Academy of Medical SciencesAbstract Background The current detection of fetal chromosomal abnormalities by non-invasive prenatal testing (NIPT) mainly relies on the cell free DNA(cfDNA) in the maternal blood. However, a gestational age of less than 12 weeks or a high maternal BMI affects cfDNA fetal fraction and further the detection by NIPT negatively. In this study, we aim to retrieve the trophoblast cells from the maternal cervix to develop a new sampling method for NIPT enabling an earlier use of NIPT. Methods We enrolled three patients who wanted to undergo induced abortion at Beijing Hospital between January 2022 and March 2022. Peripheral blood, cervix specimen, and the abortion tissue were collected and processed for each patient. Allele frequencies of the mutated gene loci of the maternal blood and the cervix sample were compared and the Sex Determining Region Y (SRY) gene was tested. Results The allele frequencies of the mutated gene loci showed no significant difference between the maternal blood and the cervix sample. But we successfully detected signal of the SRY gene in the cervix sample of the only patient carrying a male fetus. Conclusions The detection of the SRY gene in a cervix sample indicated a successful retrieval of trophoblast cells from the cervix canal. Further study needs to be conducted to verify our finding before its application to the clinical settings.https://doi.org/10.1186/s12884-023-05499-4Non-invasive prenatal testingNIPTcfDNATrophoblastCervix |
| spellingShingle | Xiaoke Yang Liuyezi Du Yue Li Lin Liang Linlin Ma Shaowei Wang Trophoblast retrieval from the cervical canal to predict abnormal pregnancy early in gestation: a pilot study BMC Pregnancy and Childbirth Non-invasive prenatal testing NIPT cfDNA Trophoblast Cervix |
| title | Trophoblast retrieval from the cervical canal to predict abnormal pregnancy early in gestation: a pilot study |
| title_full | Trophoblast retrieval from the cervical canal to predict abnormal pregnancy early in gestation: a pilot study |
| title_fullStr | Trophoblast retrieval from the cervical canal to predict abnormal pregnancy early in gestation: a pilot study |
| title_full_unstemmed | Trophoblast retrieval from the cervical canal to predict abnormal pregnancy early in gestation: a pilot study |
| title_short | Trophoblast retrieval from the cervical canal to predict abnormal pregnancy early in gestation: a pilot study |
| title_sort | trophoblast retrieval from the cervical canal to predict abnormal pregnancy early in gestation a pilot study |
| topic | Non-invasive prenatal testing NIPT cfDNA Trophoblast Cervix |
| url | https://doi.org/10.1186/s12884-023-05499-4 |
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