Fusion primer and nested integrated PCR (<it>FPNI-PCR</it>): a new high-efficiency strategy for rapid chromosome walking or flanking sequence cloning
<p>Abstract</p> <p>Background</p> <p>The advent of genomics-based technologies has revolutionized many fields of biological enquiry. However, chromosome walking or flanking sequence cloning is still a necessary and important procedure to determining gene structure. Such...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2011-11-01
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| Series: | BMC Biotechnology |
| Online Access: | http://www.biomedcentral.com/1472-6750/11/109 |