Escherichia coli in vitro recombinant system transports FhaB mutants

Objective To explore the mechanism of two-partner secretion with an in vitro reconstitution system. Methods Spheroplasts were used to construct FhaB mutants with different molecular weights, and then combined with FhaC containing proteoliposomes to perform the translocation experiments followed by d...

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Bibliographic Details
Main Author: HUANG Pi-ying, CAI Kun, WEI Liang-wan, WANG Xing-yuan, WANG Zhe, CHU Yin-di, FAN En-guo
Format: Article
Language:zho
Published: Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College. 2022-04-01
Series:Jichu yixue yu linchuang
Subjects:
Online Access:http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2022-42-4-565.pdf