Increase in <i>TPSB2</i> and <i>TPSD1</i> Expression in Synovium of Hip Osteoarthritis Patients Who Are Overweight
While research suggests that increasing body mass index (BMI) is a risk factor for hip osteoarthritis (HOA), the mechanisms of this effect are not fully understood. Tryptases are among the main proteases found in mast cells (MCs) and contribute to OA pathology. <i>TPSB2</i>, which encode...
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MDPI AG
2023-07-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/14/11532 |
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| author | Maho Tsuchiya Kensuke Fukushima Ken Takata Yoshihisa Ohashi Katsufumi Uchiyama Naonobu Takahira Hiroki Saito Ayumi Tsukada Gen Inoue Masashi Takaso Kentaro Uchida |
| author_facet | Maho Tsuchiya Kensuke Fukushima Ken Takata Yoshihisa Ohashi Katsufumi Uchiyama Naonobu Takahira Hiroki Saito Ayumi Tsukada Gen Inoue Masashi Takaso Kentaro Uchida |
| author_sort | Maho Tsuchiya |
| collection | DOAJ |
| description | While research suggests that increasing body mass index (BMI) is a risk factor for hip osteoarthritis (HOA), the mechanisms of this effect are not fully understood. Tryptases are among the main proteases found in mast cells (MCs) and contribute to OA pathology. <i>TPSB2</i>, which encodes β-tryptase, is increased in the synovium of overweight and obese knee OA patients. However, it remains unclear whether tryptase in the synovium of HOA is increased with increasing BMI. Here, we investigated tryptase genes (<i>TPSB2</i> and <i>TPSD1</i>) in the synovium of overweight HOA patients. Forty-six patients radiographically diagnosed with HOA were allocated to two groups based on BMI, namely normal (<25 kg/m<sup>2</sup>) and overweight (25–29.99 kg/m<sup>2</sup>). <i>TPSB2</i> and <i>TPSD1</i> expression in the synovium of the two groups was compared using real-time polymerase chain reaction. To compare <i>TPSB2</i> and <i>TPSD1</i> expression in MCs between the groups, we isolated the MC-rich fraction (MC-RF) and MC-poor fraction (MC-PF), extracted using magnetic isolation. <i>TPSB2</i> and <i>TPSD1</i> expression was increased in the overweight group compared with the normal group. Expression of both genes in the MC-RF was significantly higher than that in MC-PF in both groups. However, <i>TPSB2</i> and <i>TPSD1</i> expression levels in the MC-RF did not differ between the groups. Tryptase genes were highly expressed in the synovium of overweight HOA patients. Further investigation to reveal the role of tryptase in the relationship between increasing BMI and HOA pathology is required. |
| first_indexed | 2024-03-11T01:00:46Z |
| format | Article |
| id | doaj.art-802b0e4a7cbb430a88a0d6d094d0cd38 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T01:00:46Z |
| publishDate | 2023-07-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-802b0e4a7cbb430a88a0d6d094d0cd382023-11-18T19:40:59ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-07-0124141153210.3390/ijms241411532Increase in <i>TPSB2</i> and <i>TPSD1</i> Expression in Synovium of Hip Osteoarthritis Patients Who Are OverweightMaho Tsuchiya0Kensuke Fukushima1Ken Takata2Yoshihisa Ohashi3Katsufumi Uchiyama4Naonobu Takahira5Hiroki Saito6Ayumi Tsukada7Gen Inoue8Masashi Takaso9Kentaro Uchida10Department of Orthopedic Surgery, Kitasato University School of Medicine, 1-15-1 Kitasato, Minami-ku, Sagamihara City 252-0374, JapanDepartment of Orthopedic Surgery, Kitasato University School of Medicine, 1-15-1 Kitasato, Minami-ku, Sagamihara City 252-0374, JapanDepartment of Orthopedic Surgery, Kitasato University School of Medicine, 1-15-1 Kitasato, Minami-ku, Sagamihara City 252-0374, JapanDepartment of Orthopedic Surgery, Kitasato University School of Medicine, 1-15-1 Kitasato, Minami-ku, Sagamihara City 252-0374, JapanDepartment of Patient Safety and Healthcare Administration, Kitasato University School of Medicine, 1-15-1 Kitasato, Minami-ku, Sagamihara City 252-0374, JapanDepartment of Rehabilitation, Kitasato University School of Allied Health Sciences, 1-15-1 Kitasato, Minami-ku, Sagamihara City 252-0373, JapanDepartment of Orthopedic Surgery, Kitasato University School of Medicine, 1-15-1 Kitasato, Minami-ku, Sagamihara City 252-0374, JapanDepartment of Orthopedic Surgery, Kitasato University School of Medicine, 1-15-1 Kitasato, Minami-ku, Sagamihara City 252-0374, JapanDepartment of Orthopedic Surgery, Kitasato University School of Medicine, 1-15-1 Kitasato, Minami-ku, Sagamihara City 252-0374, JapanDepartment of Orthopedic Surgery, Kitasato University School of Medicine, 1-15-1 Kitasato, Minami-ku, Sagamihara City 252-0374, JapanDepartment of Orthopedic Surgery, Kitasato University School of Medicine, 1-15-1 Kitasato, Minami-ku, Sagamihara City 252-0374, JapanWhile research suggests that increasing body mass index (BMI) is a risk factor for hip osteoarthritis (HOA), the mechanisms of this effect are not fully understood. Tryptases are among the main proteases found in mast cells (MCs) and contribute to OA pathology. <i>TPSB2</i>, which encodes β-tryptase, is increased in the synovium of overweight and obese knee OA patients. However, it remains unclear whether tryptase in the synovium of HOA is increased with increasing BMI. Here, we investigated tryptase genes (<i>TPSB2</i> and <i>TPSD1</i>) in the synovium of overweight HOA patients. Forty-six patients radiographically diagnosed with HOA were allocated to two groups based on BMI, namely normal (<25 kg/m<sup>2</sup>) and overweight (25–29.99 kg/m<sup>2</sup>). <i>TPSB2</i> and <i>TPSD1</i> expression in the synovium of the two groups was compared using real-time polymerase chain reaction. To compare <i>TPSB2</i> and <i>TPSD1</i> expression in MCs between the groups, we isolated the MC-rich fraction (MC-RF) and MC-poor fraction (MC-PF), extracted using magnetic isolation. <i>TPSB2</i> and <i>TPSD1</i> expression was increased in the overweight group compared with the normal group. Expression of both genes in the MC-RF was significantly higher than that in MC-PF in both groups. However, <i>TPSB2</i> and <i>TPSD1</i> expression levels in the MC-RF did not differ between the groups. Tryptase genes were highly expressed in the synovium of overweight HOA patients. Further investigation to reveal the role of tryptase in the relationship between increasing BMI and HOA pathology is required.https://www.mdpi.com/1422-0067/24/14/11532hip osteoarthritismast cellobesitytryptase |
| spellingShingle | Maho Tsuchiya Kensuke Fukushima Ken Takata Yoshihisa Ohashi Katsufumi Uchiyama Naonobu Takahira Hiroki Saito Ayumi Tsukada Gen Inoue Masashi Takaso Kentaro Uchida Increase in <i>TPSB2</i> and <i>TPSD1</i> Expression in Synovium of Hip Osteoarthritis Patients Who Are Overweight International Journal of Molecular Sciences hip osteoarthritis mast cell obesity tryptase |
| title | Increase in <i>TPSB2</i> and <i>TPSD1</i> Expression in Synovium of Hip Osteoarthritis Patients Who Are Overweight |
| title_full | Increase in <i>TPSB2</i> and <i>TPSD1</i> Expression in Synovium of Hip Osteoarthritis Patients Who Are Overweight |
| title_fullStr | Increase in <i>TPSB2</i> and <i>TPSD1</i> Expression in Synovium of Hip Osteoarthritis Patients Who Are Overweight |
| title_full_unstemmed | Increase in <i>TPSB2</i> and <i>TPSD1</i> Expression in Synovium of Hip Osteoarthritis Patients Who Are Overweight |
| title_short | Increase in <i>TPSB2</i> and <i>TPSD1</i> Expression in Synovium of Hip Osteoarthritis Patients Who Are Overweight |
| title_sort | increase in i tpsb2 i and i tpsd1 i expression in synovium of hip osteoarthritis patients who are overweight |
| topic | hip osteoarthritis mast cell obesity tryptase |
| url | https://www.mdpi.com/1422-0067/24/14/11532 |
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