Improving an rRNA depletion protocol with statistical design of experiments
In prokaryotic RNA-seq library preparation, rRNA depletion is required to remove highly abundant rRNA transcripts from total RNA. rRNA is so abundant that small improvements in depletion efficiency lead to large increases in mRNA sequencing coverage. The current gold-standard method for rRNA depleti...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-02-01
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| Series: | SLAS Technology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2472630322051767 |